Embryonic Thermal Manipulation Affects Ventilation, Metabolism, Thermal Control and Central Dopamine in Newly Hatched and Juvenile Chicks

Aline C G Rocha; Caroline Cristina-Silva; Camila L Taxini; Kaoma Stephani da Costa Silva; Virgínia T M Lima; Marcos Macari; Kênia C Bícego; Raphael E Szawka; Luciane H Gargaglioni

doi:10.3389/fphys.2021.699142

Embryonic Thermal Manipulation Affects Ventilation, Metabolism, Thermal Control and Central Dopamine in Newly Hatched and Juvenile Chicks

Front Physiol. 2021 Jun 17:12:699142. doi: 10.3389/fphys.2021.699142. eCollection 2021.

Authors

Aline C G Rocha¹, Caroline Cristina-Silva¹, Camila L Taxini², Kaoma Stephani da Costa Silva³, Virgínia T M Lima³, Marcos Macari¹, Kênia C Bícego¹, Raphael E Szawka³, Luciane H Gargaglioni¹

Affiliations

¹ Department of Animal Morphology and Physiology, College of Agricultural and Veterinarian Sciences, São Paulo State University, São Paulo, Brazil.
² State University of Minas Gerais - UEMG, Passos, Brazil.
³ Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais - UFMG, Belo Horizonte, Brazil.

Abstract

The first third of incubation is critical for embryonic development, and environmental changes during this phase can affect the physiology and survival of the embryos. We evaluated the effects of low (LT), control (CT), and high (HT) temperatures during the first 5 days of incubation on ventilation ( $\dot{V}$ _E ), body temperature (Tb), oxygen consumption ( $\dot{V}$ O₂), respiratory equivalent ( $\dot{V}$ _E / $\dot{V}$ O₂), and brain monoamines on 3-days-old (3d) and 14-days-old (14d) male and female chickens. The body mass of LT animals of both ages and sexes was higher compared to HT and CT animals (except for 3d males). The heart mass of 14d HT animals was higher than that of CT animals. Thermal manipulation did not affect $\dot{V}$ _E , $\dot{V}$ O₂ or $\dot{V}$ _E / $\dot{V}$ O₂ of 3d animals in normoxia, except for 3d LT males $\dot{V}$ _E , which was lower than CT. Regarding 14d animals, the HT females showed a decrease in $\dot{V}$ _E and $\dot{V}$ O₂ compared to CT and LT groups, while the HT males displayed a lower $\dot{V}$ O₂ compared to CT males, but no changes in $\dot{V}$ _E / $\dot{V}$ O₂. Both sexes of 14d HT chickens presented a greater Tb compared to CT animals. Thermal manipulations increased the dopamine turnover in the brainstem of 3d females. No differences were observed in ventilatory and metabolic parameters in the 3d animals of either sexes, and 14d males under 7% CO₂. The hypercapnic hyperventilation was attenuated in the 14d HT females due to changes in $\dot{V}$ O₂, without alterations in $\dot{V}$ _E . The 14d LT males showed a lower $\dot{V}$ _E , during hypercapnia, compared to CT, without changes in $\dot{V}$ O₂, resulting in an attenuation in $\dot{V}$ _E / $\dot{V}$ O₂. During hypoxia, 3d LT females showed an attenuated hyperventilation, modulated by a higher $\dot{V}$ O₂. In 14d LT and HT females, the increase in $\dot{V}$ _E was greater and the hypometabolic response was attenuated, compared to CT females, which resulted in no change in the $\dot{V}$ _E / $\dot{V}$ O₂. In conclusion, thermal manipulations affect hypercapnia-induced hyperventilation more so than hypoxic challenge, and at both ages, females are more affected by thermal manipulation than males.

Keywords: chicken; hypercapnia; hypoxia; incubation; monoamines; temperature; ventilation.