Using glycerin with chitosan extracted from shrimp residue to enhance rumen fermentation and feed use in native Thai bulls

Anuthida Seankamsorn; Anusorn Cherdthong; Sarong So; And Metha Wanapat

doi:10.14202/vetworld.2021.1158-1164

Using glycerin with chitosan extracted from shrimp residue to enhance rumen fermentation and feed use in native Thai bulls

Vet World. 2021 May;14(5):1158-1164. doi: 10.14202/vetworld.2021.1158-1164. Epub 2021 May 12.

Authors

Anuthida Seankamsorn¹, Anusorn Cherdthong¹, Sarong So¹, And Metha Wanapat¹

Affiliation

¹ Department of Animal Science, Tropical Feed Resources Research and Development Center, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand.

Abstract

Background and aim: Crude glycerin is changed to propionate in the rumen, while chitosan can be used as a feed supplement to increase propionic acid concentration and decrease methane (CH₄) production. We hypothesized that supplementation with a combination of a high level of crude glycerin with chitosan could have a beneficial effect on ruminal fermentation and mitigate CH₄ production. This study aimed to explore the combined effects of crude glycerin and chitosan supplementation on nutrient digestibility, rumen fermentation, and CH₄ calculation in native Thai bulls.

Materials and methods: Four 2-year-old native Thai bulls, weighing 150±20 kg, were kept in a 2×2 factorial arrangement in a 4×4 Latin square design. Factor A represented the incorporation of crude glycerin at 10.5% and 21% of the dry matter (DM) of a total mixed ration (TMR), and factor B represented the supplementation of chitosan at 1% and 2% DM of a TMR.

Results: Increasing levels of crude glycerin at 21% decreased DM intake by 0.62 kg/day compared with 10.5% crude glycerin (p<0.05), whereas nutrient digestibility did not change (p>0.05). The incorporated crude glycerin and supplemented chitosan levels did not affect the pH, temperature, concentrations of ammonia-nitrogen, microbial population, and blood urea nitrogen (p>0.05). Supplemented chitosan and incorporated crude glycerin did not show any interaction effects on the molar portions and total volatile fatty acids (VFAs), except estimated CH₄. Increasing the incorporated crude glycerin levels increased propionate and decreased the ratio of acetate to propionate ratio, whereas levels of butyrate, acetate, and total VFAs were unchanged. The combination of crude glycerin at 21% in the TMR with chitosan at 2% reduced CH₄ estimation by 5.08% compared with the other feed treatment.

Conclusion: Increasing incorporated crude glycerin levels in a TMR significantly elevated the propionate concentration, whereas combining 21% crude glycerin in the TMR diet with 2% chitosan supplementation could depress CH₄ estimation more effectively than adding one of these supplements alone.

Keywords: crude glycerin; digestibility; incorporation; intake; volatile fatty acids.