Dissecting the plant chromatin interactome using mass spectrometry

Yanmei Chen; Jun Xiao; Peng Liu

doi:10.1016/j.tibtech.2021.06.002

Dissecting the plant chromatin interactome using mass spectrometry

Trends Biotechnol. 2022 Mar;40(3):261-265. doi: 10.1016/j.tibtech.2021.06.002. Epub 2021 Jun 29.

Authors

Yanmei Chen¹, Jun Xiao², Peng Liu³

Affiliations

¹ State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, 100193 Beijing, China. Electronic address: chenyanmei@cau.edu.cn.
² State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, 100101 Beijing, China.
³ KAUST Environmental Epigenetics Program, Division of Biological Environmental Sciences and Engineering, King Abdullah University of Science and Technology (KAUST), 23955-6900 Thuwal, Saudi Arabia.

PMID: 34215438
DOI: 10.1016/j.tibtech.2021.06.002

Abstract

The protein interactome mediates crucial functions in transcription, chromatin remodeling, and higher-order structural organization. Elucidating the proteins that interact with chromatin-associated RNA or proteins is key to understanding fundamental epigenetic regulatory pathways. We discuss the opportunities and challenges of mass spectrometry (MS)-based proteomics for characterizing the plant chromatin interactome.

Keywords: RNA-binding protein; chromatin; epigenetics; interactome; proteomics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatin*
Epigenomics
Mass Spectrometry / methods
Proteomics* / methods

Substances

Chromatin