Repair calvarial defect of osteoporotic rats by berberine functionalized porous calcium phosphate scaffold

Dahao Wang; Peng Zhang; Xifan Mei; Zhenhua Chen

doi:10.1093/rb/rbab022

Repair calvarial defect of osteoporotic rats by berberine functionalized porous calcium phosphate scaffold

Regen Biomater. 2021 Jun 1;8(3):rbab022. doi: 10.1093/rb/rbab022. eCollection 2021 Jun.

Authors

Dahao Wang¹, Peng Zhang², Xifan Mei², Zhenhua Chen²

Affiliations

¹ Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China.
² Jinzhou Medical University, Jinzhou 121001, China.

Abstract

In this article, we propose a simple scheme of using berberine (BBR) to modify porous calcium phosphate ceramics (named PCPC). These BBR molecules regulate the crystallization of hydroxyapatite nanorods on PCPC. We found that these nanorods and the adsorbed BBR changed the interface micro-environment of PCPC by SEM images. The microenvironment of PCPC surface is essential for promoting BMSCs' proliferation and differentiation. These results demonstrated that PCPC/BBR markedly improved the bone regeneration of osteoporosis rats. Moreover, PCPC/BBR had significantly increased the expression levels of ALP, osteocalcin and bone morphogenetic protein2 and RUNX2 in BMSCs originated from osteoporosis rats.

Keywords: BMSCs; berberine; osteoporosis; porous ceramics.