miR-302d Competitively Binding with the lncRNA-341 Targets TLE4 in the Process of SSC Generation

Yani Zhang; Wenhui Zhang; Cai Hu; Yingjie Wang; Man Wang; Qisheng Zuo; Ahmed Kamel Elsayed; Yi Li; Bichun Li

doi:10.1155/2021/5546936

miR-302d Competitively Binding with the lncRNA-341 Targets TLE4 in the Process of SSC Generation

Stem Cells Int. 2021 Jun 8:2021:5546936. doi: 10.1155/2021/5546936. eCollection 2021.

Authors

Yani Zhang^{1

2}, Wenhui Zhang^{1

2}, Cai Hu^{1

2}, Yingjie Wang^{1

2}, Man Wang^{1

2}, Qisheng Zuo^{1

2}, Ahmed Kamel Elsayed³, Yi Li⁴, Bichun Li^{1

2}

Affiliations

¹ College of Animal Science and Technology, Yangzhou University, Jiangsu Province Key Laboratory of Animal Breeding and Molecular Design, Yangzhou, 225009 Jiangsu, China.
² Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, 225009 Jiangsu, China.
³ Faculty of Veterinary Medicine, Suez Canal University, 41522 Ismailia, Egypt.
⁴ College of Computer Science and Technology, Wenzhou-Kean University, Wenzhou, 325035 Zhejiang, China.

Abstract

MicroRNAs (miRNAs) are essential factors in the reproductive process of poultry. Here, we found miR-302d is a potential differentiation and negative factor of chicken embryonic stem cells (ESCs) into spermatogonia stem cells (SSCs). The competition mechanism was carried out for the preliminary exploration to determine the relationship among miR-302d, lncRNA-341(interacting with miR-302d), and target gene TLE4. The results showed that lncRNA-341 can competitively bind to miR-302d to decrease the targeted binding of miR-302d and TLE4 which promotes the differentiation of chicken SSCs. Moreover, it is suggested that miR-302d may participate in the Wnt signaling pathway through TLE4.