DNA-Directed Assembly of Carbon Nanotube-Protein Hybrids

Mark Freeley; Rebecca E A Gwyther; D Dafydd Jones; Matteo Palma

doi:10.3390/biom11070955

DNA-Directed Assembly of Carbon Nanotube-Protein Hybrids

Biomolecules. 2021 Jun 29;11(7):955. doi: 10.3390/biom11070955.

Authors

Mark Freeley¹, Rebecca E A Gwyther², D Dafydd Jones², Matteo Palma¹

Affiliations

¹ Department of Chemistry, Queen Mary University of London, London E1 4NS, UK.
² Molecular Biosciences Division, School of Biosciences, Cardiff University, Cardiff CF10 3AX, UK.

Abstract

Here, we report the controlled assembly of SWCNT-GFP hybrids employing DNA as a linker. Two distinct, enriched SWCNTs chiralities, (6,5), (7,6), and an unsorted SWCNT solution, were selectively functionalized with DNA and hybridized to a complementary GFP^DNA conjugate. Atomic force microscopy images confirmed that GFP attachment occurred predominantly at the terminal ends of the nanotubes, as designed. The electronic coupling of the proteins to the nanotubes was confirmed via in-solution fluorescence spectroscopy, that revealed an increase in the emission intensity of GFP when linked to the CNTs.

Keywords: DNA; GFP; SWCNT; hybrid; protein; single-walled carbon nanotube.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Biosensing Techniques / methods*
DNA / chemistry*
Microscopy, Atomic Force / methods
Nanotubes, Carbon / chemistry*
Proteins / chemistry*

DNA-Directed Assembly of Carbon Nanotube-Protein Hybrids

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