Microbial electrosynthesis is a promising electricity-driven technology for converting carbon dioxide into value-added compounds, but the formation of cathodic electroactive-biofilms (CEBs) is challenging. Herein, we have demonstrated an innovative strategy for CEBs assembly via magnetic nanoparticle bioconjugation, which lies in the synergistic interactions among a bonder (Streptavidin, SA), conductive nanomaterials (Fe3O4), and a methanogen (M. barkeri). The results showed that the bioconjugated M. barkeri-SA-Fe3O4 biohybrids significantly enhanced both methane yield (33.2-fold) and faradaic efficiency (5.6-fold), compared with that of bare M. barkeri. Such an enhancement was attributed to the improved viability of CEBs with a higher biomass density. Particularly, more live cells were presented in the inner biofilms and promoted the long-distance electron exchange between the live outer-layer biofilm and the cathode electrode. Meanwhile, the higher redox activity of CEBs with the M. barkeri-SA-Fe3O4 biohybrids resulted in an improved transient charge storage capability, which was beneficial for the biological CO2-to-CH4 conversion via acting as an additional electron donor. This work has provided a new approach to accelerate the formation of CEBs and subsequent electron transfer, which holds a great potential for accomplishing electrosynthesis and CO2 fixation.
Keywords: Carbon dioxide reduction; Electrosynthesis; Magnetic streptavidin; Methane production; Redox activity.
Copyright © 2021 Elsevier B.V. All rights reserved.