Expression of soluble recombinant human matrix metalloproteinase 9 and generation of its monoclonal antibody

Hee-Jin Jeong; Eun-Jung Kim; Joo-Kyung Kim; Yun-Gon Kim; Chang-Soo Lee; Byoung Joon Ko; Byung-Gee Kim

doi:10.1016/j.pep.2021.105931

Expression of soluble recombinant human matrix metalloproteinase 9 and generation of its monoclonal antibody

Protein Expr Purif. 2021 Nov:187:105931. doi: 10.1016/j.pep.2021.105931. Epub 2021 Jun 29.

Authors

Hee-Jin Jeong¹, Eun-Jung Kim², Joo-Kyung Kim³, Yun-Gon Kim⁴, Chang-Soo Lee⁵, Byoung Joon Ko⁶, Byung-Gee Kim⁷

Affiliations

¹ Department of Biological and Chemical Engineering, Hongik University, Sejong, South Korea. Electronic address: heejinjeong@hongik.ac.kr.
² Bio-MAX/N-Bio, Seoul National University, Seoul, South Korea.
³ Department of Biological and Chemical Engineering, Hongik University, Sejong, South Korea.
⁴ Department of Chemical Engineering, Soongsil University, Seoul, South Korea.
⁵ Department of Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon, South Korea.
⁶ School of Biopharmaceutical and Medical Sciences, Sungshin Women's University, Seoul, South Korea.
⁷ Bio-MAX/N-Bio, Seoul National University, Seoul, South Korea; School of Chemical and Biological Engineering, Seoul National University, Seoul, South Korea. Electronic address: byungkim@snu.ac.kr.

PMID: 34197919
DOI: 10.1016/j.pep.2021.105931

Abstract

We have successfully produced a recombinant human matrix metalloproteinase 9 (hMMP9) antigen with high yield and purity and used it to generate a hybridoma cell-culture-based monoclonal anti-hMMP9 antibody. We selected the most effective antibody for binding antigens and successfully identified its nucleotide sequence. The entire antigen and antibody developmental procedures described herein can be a practical approach for producing large amounts of monoclonal antibodies against hMMP9 and other antigens of interest. Additionally, the nucleotide sequence information of the anti-hMMP9 monoclonal antibody revealed herein will be useful for the generation of recombinant antibodies or antibody fragments against hMMP9.

Keywords: Hybridoma technology; Matrix metalloproteinase 9; Monoclonal antibody; Recombinant protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Monoclonal / chemistry
Antibodies, Monoclonal / genetics*
Antibodies, Monoclonal / immunology
Base Sequence
Cell Culture Techniques
Gene Expression Regulation
Humans
Hybridomas / cytology
Immunoglobulin Fragments / chemistry
Matrix Metalloproteinase 9 / chemistry
Matrix Metalloproteinase 9 / genetics*
Matrix Metalloproteinase 9 / immunology
Protein Binding
Recombinant Proteins / chemistry
Recombinant Proteins / genetics*
Recombinant Proteins / immunology
Solubility

Substances

Antibodies, Monoclonal
Immunoglobulin Fragments
Recombinant Proteins
MMP9 protein, human
Matrix Metalloproteinase 9