Isolation, characterization and in vitro cytotoxicity studies of bioactive compounds from Alseodaphne semecarpifolia Nees

Ganadhal Puttaramaiah Chethankumara; Kakanahalli Nagaraj; Venkatarangaiah Krishna; Gurunathan Krishnaswamy

doi:10.1016/j.heliyon.2021.e07325

Isolation, characterization and in vitro cytotoxicity studies of bioactive compounds from Alseodaphne semecarpifolia Nees

Heliyon. 2021 Jun 15;7(6):e07325. doi: 10.1016/j.heliyon.2021.e07325. eCollection 2021 Jun.

Authors

Ganadhal Puttaramaiah Chethankumara¹, Kakanahalli Nagaraj¹, Venkatarangaiah Krishna², Gurunathan Krishnaswamy³

Affiliations

¹ Department of PG Studies and Research in Applied Zoology, Kuvempu University, Shivamogga, Karnataka, 577 451, India.
² Department of PG Studies and Research in Biotechnology, Kuvempu University, Shivamogga, Karnataka, 577 451, India.
³ Department of PG Studies and Research in Organic Chemistry, Tumkur University, Tumakuru, Karnataka, 572 102, India.

Abstract

Alseodaphne semecarpifolia (Lauraceae) is a traditional medicinal plant historically used in Indian Western Ghats to treat various human diseases. From the past few decades it has been traditionally recognized as an effective agent in cancer treatment. The phytochemical investigation of the stem bark and leaves of A. semecarpifolia led to the isolation of bioactive flavonoid compounds Icariin and Baicalein. Their structures were elucidated from obtained spectral data (¹H NMR, ¹³C NMR, ¹H ¹H COSY, FTIR and MS). Cytotoxic activity of Icariin and Baicalein evaluated against MCF-7 cells revealed their potent activity with an IC₅₀ of 42.15 ± 4.78 μg/ml and 44.37 ± 3.46 μg/ml, respectively, while very least effect was observed on normal cells (L6). Present study has suggested that both Icariin and Baicalein have potent cytotoxic activity against MCF-7 cells.

Keywords: Alseodaphne semecarpifolia; Baicalein; Cytotoxicity; Icariin; MCF-7.