Gene expression of cytokinesis regulators PRC1, KIF14 and CIT has no prognostic role in colorectal and pancreatic cancer

Vojtech Hanicinec; Veronika Brynychova; Jachym Rosendorf; Richard Palek; Vaclav Liska; Martin Oliverius; Zdenek Kala; Beatrice Mohelnikova-Duchonova; Ivona Krus; Pavel Soucek

doi:10.3892/ol.2021.12859

Gene expression of cytokinesis regulators PRC1, KIF14 and CIT has no prognostic role in colorectal and pancreatic cancer

Oncol Lett. 2021 Aug;22(2):598. doi: 10.3892/ol.2021.12859. Epub 2021 Jun 9.

Authors

Vojtech Hanicinec¹, Veronika Brynychova¹, Jachym Rosendorf^{1

2}, Richard Palek^{1

2}, Vaclav Liska^{1

2}, Martin Oliverius³, Zdenek Kala⁴, Beatrice Mohelnikova-Duchonova⁵, Ivona Krus⁶, Pavel Soucek^{1

6}

Affiliations

¹ Biomedical Centre, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech Republic.
² Deparment of Surgery, Teaching Hospital and Faculty of Medicine in Pilsen, Charles University, 30460 Pilsen, Czech Republic.
³ Department of Surgery, Faculty Hospital Kralovske Vinohrady and Third Faculty of Medicine, Charles University, 10000 Prague, Czech Republic.
⁴ Department of Surgery, University Hospital Brno and Faculty of Medicine, Masaryk University, 62500 Brno, Czech Republic.
⁵ Department of Oncology and Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, 77900 Olomouc, Czech Republic.
⁶ Department of Toxicogenomics, National Institute of Public Health, Prague 10042, Czech Republic.

Abstract

Colorectal cancer is one of the most common cancers and pancreatic cancer is among the most fatal and difficult to treat. New prognostic biomarkers are urgently needed to improve the treatment of colorectal and pancreatic cancer. Protein regulating cytokinesis 1 (PRC1), kinesin family member 14 (KIF14) and citron Rho-interacting serine/threonine kinase (CIT) serve important roles in cytokinesis, are strongly associated with cancer progression and have prognostic potential. The present study aimed to investigate the prognostic relevance of the PRC1, KIF14 and CIT genes in colorectal and pancreatic cancer. PRC1, KIF14 and CIT transcript expression was assessed by reverse transcription-quantitative PCR in tumors and paired distant unaffected mucosa from 67 patients with colorectal cancer and tumors and paired non-neoplastic control tissues from 48 patients with pancreatic cancer. The extent of transcript dysregulation between tumor and control tissues and between groups of patients divided by main clinical characteristics, namely patients' age and sex, disease stage, localization and grade, was determined. Finally, the associations of transcript levels in tumors with disease-free interval and overall survival time were evaluated. PRC1, KIF14 and CIT transcripts were upregulated in tumors compared with control tissues. PRC1, KIF14 and CIT levels strongly correlated to each other in both colorectal and pancreatic tumor and control tissues after correction for multiple testing. However, no significant associations were found among the transcript levels of PRC1, KIF14 and CIT and disease-free interval or overall survival time. In summary, the present study demonstrated mutual correlation of PRC1, KIF14 and CIT cytokinesis regulators with no clear prognostic value in pancreatic and colorectal cancers. Hence, according to the results of the present study, transcript levels of these genes cannot be clinically exploited as prognostic biomarkers in colorectal or pancreatic cancer patients.

Keywords: cancer; colon; cytokinesis; gene expression; pancreas; prognosis; rectum.

Grants and funding

This work was supported by the Czech Medical Council (grant. no. NV19-08-00113 to PS), the Grant Agency of Charles University (grant. no. UNCE/MED/006 to VL), and by the Ministry of Education, Youth and Sports of the Czech Republic (grant. no. CZ.02.1.01/0.0/0.0/16_019/000 787 awarded by the MEYS CR, financed from EFRR to VB).