Proposal for reunification of the genus Raoultella with the genus Klebsiella and reclassification of Raoultella electrica as Klebsiella electrica comb. nov

Yuanyuan Ma; Xiuqin Wu; Shuying Li; Lie Tang; Mingyue Chen; Qianli An

doi:10.1016/j.resmic.2021.103851

Proposal for reunification of the genus Raoultella with the genus Klebsiella and reclassification of Raoultella electrica as Klebsiella electrica comb. nov

Res Microbiol. 2021 Sep-Oct;172(6):103851. doi: 10.1016/j.resmic.2021.103851. Epub 2021 Jun 27.

Authors

Yuanyuan Ma¹, Xiuqin Wu², Shuying Li³, Lie Tang⁴, Mingyue Chen⁵, Qianli An⁶

Affiliations

¹ Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Zhejiang Province Key Laboratory of Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China. Electronic address: 21816168@zju.edu.cn.
² Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Zhejiang Province Key Laboratory of Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China. Electronic address: 924662410@qq.com.
³ Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Zhejiang Province Key Laboratory of Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China. Electronic address: 18853817196@163.com.
⁴ Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Zhejiang Province Key Laboratory of Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China. Electronic address: tanglie23@163.com.
⁵ Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Zhejiang Province Key Laboratory of Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China. Electronic address: chenmy2007525@163.com.
⁶ Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Zhejiang Province Key Laboratory of Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China. Electronic address: an@zju.edu.cn.

PMID: 34186167
DOI: 10.1016/j.resmic.2021.103851

Abstract

The order Enterobacterales was divided into seven families including the family Enterobacteriaceae in 2016. The genus Klebsiella within the family Enterobacteriaceae was divided into two genera Klebsiella and Raoultella in 2001. Here, our phylogenomic analysis shows that the genus Raoultella is nested within the genus Klebsiella. Klebsiella and Raoultella together are monophyletic and share average amino acid identities (AAIs) of 86.9-89.6% above the AAI threshold (86%) for genus delimitation within the family Enterobacteriaceae. Klebsiella and Raoultella share AAIs of 79.9%-85.0% with the other genera within the subfamily "Klebsiella clade", which are in the range of inter-genus AAIs (74-85%) within the family Enterobacteriaceae. Klebsiella and Raoultella also share six known conserved signature indels. Therefore, we propose to reunify Klebsiella and Raoultella to the single genus Klebsiella and reclassify Raoultella electrica as Klebsiella electrica comb. nov. Our genome-based taxonomic analyses also identified seven potential novel species within the unified genus Klebsiella.

Keywords: Average amino acid identity; Beta-lactamase; Enterobacteriaceae; Taxogenomics.

MeSH terms

Amino Acid Sequence
Bacterial Proteins / chemistry
Bacterial Typing Techniques
Enterobacteriaceae / classification*
Enterobacteriaceae / genetics
Genes, rRNA
Genome, Bacterial*
INDEL Mutation
Klebsiella / classification*
Klebsiella / genetics
Klebsiella pneumoniae / classification
Klebsiella pneumoniae / genetics
Phenotype
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Terminology as Topic
Whole Genome Sequencing

Substances

Bacterial Proteins
RNA, Ribosomal, 16S

Supplementary concepts

Raoultella electrica