Complex Modulating Effects of Dietary Calcium Intake on Obese Mice

Maiko Takahashi; Takahiro Hamoya; Takumi Narita; Gen Fujii; Yukari Totsuka; Masahito Hagio; Kosuke Tashiro; Masami Komiya; Michihiro Mutoh

doi:10.21873/invivo.12480

Complex Modulating Effects of Dietary Calcium Intake on Obese Mice

In Vivo. 2021 Jul-Aug;35(4):2107-2114. doi: 10.21873/invivo.12480.

Authors

Maiko Takahashi¹, Takahiro Hamoya^{1

2}, Takumi Narita^{1

2}, Gen Fujii³, Yukari Totsuka⁴, Masahito Hagio⁵, Kosuke Tashiro⁶, Masami Komiya¹, Michihiro Mutoh^{7

2}

Affiliations

¹ Division of Prevention, Center for Public Health Sciences, National Cancer Center, Tokyo, Japan.
² Department of Molecular-Targeting Prevention, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
³ Central Radioisotope Division, National Cancer Center Research, Tokyo, Japan.
⁴ Department of Animal Experimentation, National Cancer Center Research Institute, Tokyo, Japan.
⁵ Chemistry Division, Kanagawa Prefectural Institute of Public Health, Chigasaki, Japan.
⁶ Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushuu University, Fukuoka, Japan.
⁷ Division of Prevention, Center for Public Health Sciences, National Cancer Center, Tokyo, Japan; mimutoh@koto.kpu-m.ac.jp.

Abstract

Background/aim: Οverweight and obesity are risk factors for chronic diseases. Dietary calcium has been reported to exert anti-obesity effects. However, the complex modulating effects of calcium intake on obese mice have not been clarified.

Materials and methods: The effects of calcium intake on body weight/visceral fat mass were examined in the obese mouse model, KK-A^y Results: Body weight gain decreased in mice fed a diet containing 0.4 to 3.2% calcium at the age of 11 and 13 weeks, but not at 12 weeks after normalization for food intake. Calcium intake also decreased serum insulin levels and increased the amount of feces excreted. Fecal deoxycholate levels were lower in the high-calcium group than in the normal diet control group. Furthermore, the ratio of the deoxycholate-producing microbiome in feces decreased.

Conclusion: Dietary calcium has anti-obesity effects in obese KK-A^y mice. Inhibition of insulin production and an increased amount of feces excreted with calcium intake may affect body weight.

Keywords: Dietary calcium intake; KK-Ay; deoxycholic acid; insulin; mice; visceral fat volume.

MeSH terms

Animals
Body Weight
Calcium, Dietary*
Diet
Mice
Mice, Inbred C57BL
Mice, Obese
Obesity* / etiology

Substances

Calcium, Dietary