Franz cells for facile biosensor evaluation: A case of HRP/SWCNT-based hydrogen peroxide detection via amperometric and wireless modes

Van Chinh Hoang; Atefeh Shafaat; Skaidre Jankovskaja; Vincent G Gomes; Tautgirdas Ruzgas

doi:10.1016/j.bios.2021.113420

Franz cells for facile biosensor evaluation: A case of HRP/SWCNT-based hydrogen peroxide detection via amperometric and wireless modes

Biosens Bioelectron. 2021 Nov 1:191:113420. doi: 10.1016/j.bios.2021.113420. Epub 2021 Jun 18.

Authors

Van Chinh Hoang¹, Atefeh Shafaat², Skaidre Jankovskaja², Vincent G Gomes³, Tautgirdas Ruzgas⁴

Affiliations

¹ Department of Biomedical Science, Faculty of Health and Society, Malmö University, SE-205 06, Malmö, Sweden; Biofilms - Research Center for Biointerfaces, Malmö University, SE-205 06, Malmö, Sweden; The University of Sydney, School of Chemical and Biomolecular Engineering, NSW, 2006, Australia.
² Department of Biomedical Science, Faculty of Health and Society, Malmö University, SE-205 06, Malmö, Sweden; Biofilms - Research Center for Biointerfaces, Malmö University, SE-205 06, Malmö, Sweden.
³ The University of Sydney, School of Chemical and Biomolecular Engineering, NSW, 2006, Australia. Electronic address: vincent.gomes@sydney.edu.au.
⁴ Department of Biomedical Science, Faculty of Health and Society, Malmö University, SE-205 06, Malmö, Sweden; Biofilms - Research Center for Biointerfaces, Malmö University, SE-205 06, Malmö, Sweden. Electronic address: tautgirdas.ruzgas@mau.se.

PMID: 34182432
DOI: 10.1016/j.bios.2021.113420

Abstract

Reducing animal use in biosensor research requires broader use of in vitro methods. In this work, we present a novel application of Franz cells suitable for biosensor development and evaluation in vitro. The work describes how Franz cell can be equipped with electrodes enabling characterization of biosensors in close proximity to skin. As an example of a sensor, hydrogen peroxide biosensor was prepared based on horseradish peroxidase (HRP)/single-walled carbon nanotube (SWCNT)-modified textile. The electrode exhibited lower detection limit of 0.3 μM and sensitivity of 184 μA mM^-1 cm^-2. The ability of this biosensor to monitor H₂O₂ penetration through skin and dialysis membranes was evaluated in Franz cell setup in amperometric and wireless modes. The results also show that catalase activity present in skin is a considerable problem for epidermal sensing of H₂O₂. This work highlights opportunities and obstacles that can be addressed by assessment of biosensors in Franz cell setup before progressing to their testing in animals and humans.

Keywords: Epidermal sensing; Franz cell; Hydrogen peroxide biosensor; Skin membrane.

Publication types

Case Reports

MeSH terms

Biosensing Techniques*
Electrodes
Enzymes, Immobilized
Horseradish Peroxidase
Humans
Hydrogen Peroxide
Nanotubes, Carbon*
Renal Dialysis

Substances

Enzymes, Immobilized
Nanotubes, Carbon
Hydrogen Peroxide
Horseradish Peroxidase