MicroRNA-155 influences cell damage in ischemic stroke via TLR4/MYD88 signaling pathway

Bioengineered. 2021 Dec;12(1):2449-2458. doi: 10.1080/21655979.2021.1935066.

Abstract

Cerebral ischemic stroke (CIS) is extremely harmful, and its treatment should be underpinned by understanding its pathogenic mechanism. This study was designed to determine the involvement of miR-155 in CIS development via the TLR4/MyD88 signaling pathway. First, we quantified serum miR-155 in patients with CIS and healthy individuals, and found high expression of miR-155 in such patients and a decrease in it in the patients after therapy (P < 0.05). Serum miR-155 demonstrated a favorable function in predicting the development and prognosis of CIS (P < 0.001). We also conducted a mouse assay, and found that knocking out miR-155 can improve the neurological function of mice and suppress protein TLR4 and MyD88 (all P < 0.05). Finally, we carried out a cell assay, and found enhancement in the activity of SH-SY5Y cells, decrease in their apoptosis, and protein TLR4 and MyD88 in them after suppression of miR-155 (all P < 0.05). Furthermore, we also found complete reverse by TLR4/MyD88 pathway inhibitor on the influence of increasing miR-155 on cells (P > 0.05). Therefore, with an increase in cases with CIS, miR-155 takes a part in the development of cell damage by activating TLR4/MyD88, and it is probably the key to diagnosing and treating CIS.

Keywords: MiR-155; cell damage; ischemic stroke; tlr4/myd88.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Brain / pathology
  • Cell Line, Tumor
  • Gene Expression Regulation
  • Humans
  • Ischemic Stroke / genetics*
  • Ischemic Stroke / pathology*
  • Ischemic Stroke / physiopathology
  • Memory
  • Mice
  • Mice, Inbred C57BL
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Middle Aged
  • Morris Water Maze Test
  • Myeloid Differentiation Factor 88 / metabolism*
  • Signal Transduction*
  • Spatial Learning
  • Toll-Like Receptor 4 / metabolism*

Substances

  • MIRN155 microRNA, human
  • MicroRNAs
  • Mirn155 microRNA, mouse
  • Myeloid Differentiation Factor 88
  • Toll-Like Receptor 4

Grants and funding

1.Project of clinical medical technology innovation guidance plan of Hunan Provincial Department of science and Technology (Project No.2017SK51110) 2.Project of excellent youth fund of Chenzhou first people's Hospital (Project No. N2018-002) 3.Research project of traditional Chinese medicine in Hunan Province (subject No.201966) 4.Research project of Chenzhou science and Technology Bureau (Project No.ZDYF 2020125) 5.Teaching reform project of Chenzhou first people's Hospital (Project No.CJG 2020-10)