Mouse Satellite Cell Isolation and Transplantation

Elia Angelino; Simone Reano; Michele Ferrara; Emanuela Agosti; Hana Sustova; Valeria Malacarne; Sara Clerici; Andrea Graziani; Nicoletta Filigheddu

doi:10.21769/BioProtoc.2696

Mouse Satellite Cell Isolation and Transplantation

Bio Protoc. 2018 Jan 20;8(2):e2696. doi: 10.21769/BioProtoc.2696.

Authors

Elia Angelino^{1

2

3}, Simone Reano^{1

2}, Michele Ferrara^{1

2

3}, Emanuela Agosti^{1

2}, Hana Sustova^{1

2}, Valeria Malacarne^{1

3}, Sara Clerici³, Andrea Graziani^{1

2

3}, Nicoletta Filigheddu^{1

2}

Affiliations

¹ Department of Translational Medicine, University of Piemonte Orientale, Novara, Italy.
² Istituto Interuniversitario di Miologia (IIM).
³ Division of Experimental Oncology, Università Vita-Salute San Raffaele, Milano, Italy.

Abstract

Satellite cell (SC) transplantation represents a powerful strategy to investigate SC biology during muscle regeneration. We described here a protocol for SC isolation from green fluorescent protein (GFP)-expressing mice and their transplantation into murine muscles. This procedure was originally used to assess the effects of the hormone unacylated ghrelin on muscle regeneration, in particular evaluating how the increase of unacylated ghrelin in the recipient muscle affected the engraftment of donor SCs ( Reano et al., 2017 ).

Keywords: Cardiotoxin; Engraftment; Muscle excision; Muscle stem cells; Regeneration; Satellite cells; Skeletal muscle; Transplant.