Surface-enhanced Raman scattering-based lateral flow immunoassay mediated by hydrophilic-hydrophobic Ag-modified PMMA substrate

Siqi Tang; Hongmei Liu; Yiran Tian; Dong Chen; Chenjie Gu; Guodong Wei; Tao Jiang; Jun Zhou

doi:10.1016/j.saa.2021.120092

Surface-enhanced Raman scattering-based lateral flow immunoassay mediated by hydrophilic-hydrophobic Ag-modified PMMA substrate

Spectrochim Acta A Mol Biomol Spectrosc. 2021 Dec 5:262:120092. doi: 10.1016/j.saa.2021.120092. Epub 2021 Jun 18.

Authors

Siqi Tang¹, Hongmei Liu², Yiran Tian¹, Dong Chen¹, Chenjie Gu¹, Guodong Wei³, Tao Jiang⁴, Jun Zhou¹

Affiliations

¹ Department of Microelectronic Science and Engineering, School of Physical Science and Technology, Ningbo University, Ningbo 315211, Zhejiang, PR China.
² Institute of Solid State Physics, Shanxi Datong University, Datong 037009, Shanxi, PR China.
³ Materials Institute of Atomic and Molecular Science, Shanxi University of Science and Technology, Weiyang University Park, Xian 710021, Shanxi, PR China.
⁴ Department of Microelectronic Science and Engineering, School of Physical Science and Technology, Ningbo University, Ningbo 315211, Zhejiang, PR China. Electronic address: jiangtao@nbu.edu.cn.

PMID: 34175758
DOI: 10.1016/j.saa.2021.120092

Abstract

Recently, it is urgent to ameliorate the accumulation and quantification performances of surface-enhanced Raman scattering-based lateral flow immunoassay (SERS-based LFIA) to promote its reliable clinical application. Herein, a smart hydrophilic-hydrophobic SERS-based LFIA strip was demonstrated by decorating Ag nanoplates with hydrophilic surface onto the specific regions of hydrophobic polymethylmethacrylate (PMMA) film with Raman internal standard (IS), which can unexpectedly inhibit the "coffee-ring phenomenon". The target analytes were consequently enriched in the SERS-active Ag regions by the hydrophobic PMMA, considerably endowing the strip with amended quantitative monitoring ability. Aided by immunoprobes of flower-shaped Ag nanoplates, a limit of detection as 10 pg/mL and an outstanding correlation coefficient value (R²) of 0.992 for carcinoembryonic antigen (CEA) were obtained by utilizing this SERS-based LFIA strip, which can be conducive to clinical monitoring and will broaden the field of vision for the point-of-care diagnostic technique.

Keywords: Coffee-ring; Hydrophilic-hydrophobic; Internal standard; Lateral flow immunoassay; PMMA; SERS immunoassay.

MeSH terms

Gold
Immunoassay
Limit of Detection
Metal Nanoparticles*
Polymethyl Methacrylate
Silver
Spectrum Analysis, Raman*

Substances

Silver
Gold
Polymethyl Methacrylate