Protein profile of MCF-7 breast cancer cell line treated with lectin delivered by CaCO3NPs revealed changes in molecular chaperones, cytoskeleton, and membrane-associated proteins

Rana I Mahmood; Amal Kh Abbass; Nurhanani Razali; Ali Z Al-Saffar; Jameel R Al-Obaidi

doi:10.1016/j.ijbiomac.2021.06.144

Protein profile of MCF-7 breast cancer cell line treated with lectin delivered by CaCO₃NPs revealed changes in molecular chaperones, cytoskeleton, and membrane-associated proteins

Int J Biol Macromol. 2021 Aug 1:184:636-647. doi: 10.1016/j.ijbiomac.2021.06.144. Epub 2021 Jun 24.

Authors

Rana I Mahmood¹, Amal Kh Abbass², Nurhanani Razali³, Ali Z Al-Saffar⁴, Jameel R Al-Obaidi⁵

Affiliations

¹ Department of Biology, College of Science, Baghdad University, Baghdad, Iraq; Department of Biomedical Engineering, College of Engineering, Al-Nahrain University, Baghdad, Iraq.
² Department of Biology, College of Science, Baghdad University, Baghdad, Iraq.
³ Department of Hygienic Sciences, Kobe Pharmaceutical University, Motoyamakita-machi, Higashinada-ku, 658-8558, Kobe, Japan; Membranology Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna-son, Kunigami-gun, Okinawa, Japan, 904-0495.
⁴ Department of Molecular and Medical Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq.
⁵ Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, 35900 Tanjong Malim, Perak, Malaysia. Electronic address: jr_alobaidi@yahoo.com.

PMID: 34174302
DOI: 10.1016/j.ijbiomac.2021.06.144

Abstract

The second most predominant cancer in the world and the first among women is breast cancer. We aimed to study the protein abundance profiles induced by lectin purified from the Agaricus bisporus mushroom (ABL) and conjugated with CaCO₃NPs in the MCF-7 breast cancer cell line. Two-dimensional electrophoresis (2-DE) and orbitrap mass spectrometry techniques were used to reveal the protein abundance pattern induced by lectin. Flow cytometric analysis showed the accumulation of ABL-CaCO₃NPs treated cells in the G1 phase than the positive control. Thirteen proteins were found different in their abundance in breast cancer cells after 24 h exposure to lectin conjugated with CaCO₃NPs. Most of the identified proteins were showing a low abundance in ABL-CaCO₃NPs treated cells in comparison to the positive and negative controls, including V-set and immunoglobulin domain, serum albumin, actin cytoplasmic 1, triosephosphate isomerase, tropomyosin alpha-4 chain, and endoplasmic reticulum chaperone BiP. Hornerin, tropomyosin alpha-1 chain, annexin A2, and protein disulfide-isomerase were up-regulated in comparison to the positive. Bioinformatic analyses revealed the regulation changes of these proteins mainly affected the pathways of 'Bcl-2-associated athanogene 2 signalling pathway', 'Unfolded protein response', 'Caveolar-mediated endocytosis signalling', 'Clathrin-mediated endocytosis signalling', 'Calcium signalling' and 'Sucrose degradation V', which are associated with breast cancer. We concluded that lectin altered the abundance in molecular chaperones/heat shock proteins, cytoskeletal, and metabolic proteins. Additionally, lectin induced a low abundance of MCF-7 cancer cell proteins in comparison to the positive and negative controls, including; V-set and immunoglobulin domain, serum albumin, actin cytoplasmic 1, triosephosphate isomerase, tropomyosin alpha-4 chain, and endoplasmic reticulum chaperone BiP.

Keywords: Biomarkers; Breast cancer; Lectin; Proteomics.

MeSH terms

Agaricus / chemistry*
Breast Neoplasms / drug therapy
Breast Neoplasms / metabolism*
Calcium Carbonate / chemistry*
Cell Cycle / drug effects
Cell Proliferation / drug effects
Cell Survival / drug effects
Female
Gene Expression Regulation, Neoplastic / drug effects
Gene Regulatory Networks / drug effects*
Humans
Lectins / chemistry
Lectins / pharmacology*
MCF-7 Cells
Nanoparticles
Proteomics / methods*

Substances

Lectins
Calcium Carbonate

Supplementary concepts

Agaricus bisporus