Evolutionary genomics of recent clinical Bordetella pertussis isolates from Iran: wide circulation of multiple ptxP3 lineages and report of the first ptxP3 filamentous hemagglutinin-negative B. pertussis

Azadeh Safarchi; Samaneh Saedi; Sophie Octavia; Mehdi Sedaghatpour; Negin Bolourchi; Chin Yen Tay; Binit Lamichhane; Fereshteh Shahcheraghi; Ruiting Lan

doi:10.1016/j.meegid.2021.104970

Evolutionary genomics of recent clinical Bordetella pertussis isolates from Iran: wide circulation of multiple ptxP3 lineages and report of the first ptxP3 filamentous hemagglutinin-negative B. pertussis

Infect Genet Evol. 2021 Sep:93:104970. doi: 10.1016/j.meegid.2021.104970. Epub 2021 Jun 23.

Authors

Azadeh Safarchi¹, Samaneh Saedi², Sophie Octavia³, Mehdi Sedaghatpour², Negin Bolourchi², Chin Yen Tay⁴, Binit Lamichhane⁵, Fereshteh Shahcheraghi⁶, Ruiting Lan⁷

Affiliations

¹ Pertussis Reference Laboratory, Department of Bacteriology, Pasteur Institute of Iran, Tehran 1316943551, Islamic Republic of Iran; School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia.
² Pertussis Reference Laboratory, Department of Bacteriology, Pasteur Institute of Iran, Tehran 1316943551, Islamic Republic of Iran.
³ School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia. Electronic address: s.octavia@unsw.edu.au.
⁴ Pathology and Laboratory Medicine, University of Western Australia, Perth, Western Australia 6009, Australia. Electronic address: alfred.tay@uwa.edu.au.
⁵ Pathology and Laboratory Medicine, University of Western Australia, Perth, Western Australia 6009, Australia. Electronic address: binit.lamichhane@uwa.edu.au.
⁶ Pertussis Reference Laboratory, Department of Bacteriology, Pasteur Institute of Iran, Tehran 1316943551, Islamic Republic of Iran. Electronic address: shahcheraghi@pasteur.ac.ir.
⁷ School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia. Electronic address: r.lan@unsw.edu.au.

PMID: 34171476
DOI: 10.1016/j.meegid.2021.104970

Abstract

Here we investigated nationwide clinical Bordetella pertussis isolated during 2005-2017 from different provinces of Iran, a country with more than 50 years whole cell vaccine immunisation history. Our results revealed the ongoing increase in the population of ptxP3/fim3-2 B. pertussis isolates in different provinces which were differentiated into nine clades. The largest clade (clade 8) which was previously found to be prevalent in Tehran was also prevalent across the country and clade 5 with ptxP3/prn9 genotype has also increased in frequency (14% of all ptxP3 isolates) in recent years. Furthermore, we detected the first ptxP3 B. pertussis isolates that does not express filamentous hemagglutinin (FhaB) as one of the major antigens of the pathogen and a key component of the acellular pertussis vaccine.

Keywords: Bordetella pertussis; Fha-negative B. pertussis; Iran; Phylogeny analysis; Whole genome sequencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bordetella pertussis / classification
Bordetella pertussis / genetics*
Evolution, Molecular*
Genome, Bacterial*
Hemagglutinins / immunology*
Iran
Pertussis Vaccine / genetics*
Pertussis Vaccine / immunology

Substances

Hemagglutinins
Pertussis Vaccine