Fraction collectors are common pieces of equipment that are essential for the activity of many biochemistry, pharmacology, and drug discovery laboratories. However, these devices are not very versatile when it comes to tailoring them to specific needs, such as different size collection tubes, sequences of tube exchange, or parallel collection. In addition, these systems are relatively expensive, especially for small laboratories or for those in less developed countries. The emergence of 3D printers and the availability of cheap, popular electronic control devices are changing the way laboratory equipment can be made and designed. Here, we describe how to build your own fraction collector, indicating all the elements and providing the full instructions needed to make a fraction collector that can be adapted to almost any kind of rack and tubes (3D files, the parts required, the electronic circuits, and the software). This device can be used in complex protocols, adapted to liquid chromatography and for parallel collection from perfused tissues. The total cost of the whole device is around €100.