Molecular forms of dopamine beta-hydroxylase in rat superior cervical ganglion and adrenal gland

N H Fraeyman; E J Van de Velde; F H De Smet

doi:10.1007/BF01959148

Molecular forms of dopamine beta-hydroxylase in rat superior cervical ganglion and adrenal gland

Experientia. 1988 Sep 15;44(9):746-9. doi: 10.1007/BF01959148.

Authors

N H Fraeyman¹, E J Van de Velde, F H De Smet

Affiliation

¹ Heymans Institute of Pharmacology, University of Ghent, Medical School, Belgium.

PMID: 3416990
DOI: 10.1007/BF01959148

Abstract

Dopamine beta-hydroxylase (DBH) enzyme activity was associated in rat superior cervical ganglion with tetrameric DBH-A (294,000 D) and dimeric DBH-B (147,000 D) and in rat adrenal gland with DBH-A and a novel molecular form of DBH, defined as DBH-C, with a molecular weight of 125,000 D. Pretreatment of the rats with cycloheximide markedly reduced DBH activity without altering the molecular heterogeneity.

MeSH terms

Adrenal Glands / enzymology*
Animals
Centrifugation, Density Gradient
Dopamine beta-Hydroxylase / classification
Dopamine beta-Hydroxylase / metabolism*
Ganglia, Sympathetic / enzymology*
Molecular Weight
Rats
Subcellular Fractions / enzymology

Substances

Dopamine beta-Hydroxylase