Protocol for evaluating autophagy using LysoTracker staining in the epithelial follicle stem cells of the Drosophila ovary

STAR Protoc. 2021 Jun 11;2(2):100592. doi: 10.1016/j.xpro.2021.100592. eCollection 2021 Jun 18.

Abstract

We have outlined the approach of visualizing autophagy specifically in the epithelial follicle stem cells of the Drosophila ovary using the LysoTracker dye. The advantage of using this protocol is that it details several techniques, including ovary dissection, immunofluorescence, and western blotting, that positively identify autophagy changes in a very small population of cells. One of the limitations of this protocol is that it needs to be combined with other genetic manipulations and positive markers of the autophagy pathway. For complete details on the use and execution of this protocol, please refer to Singh et al., (2018).

Keywords: Cell Biology; Genetics; Microscopy; Model Organisms; Molecular Biology; Stem Cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autophagy*
  • Cell Tracking*
  • Drosophila
  • Epithelial Cells / cytology
  • Female
  • Microscopy, Confocal
  • Ovarian Follicle / cytology*
  • Stem Cells / cytology*