Centimeter-scale wide-field-of-view laser-scanning photoacoustic microscopy for subcutaneous microvasculature in vivo

Tangyun Liao; Yuan Liu; Junwei Wu; Lijun Deng; Yu Deng; Lvming Zeng; Xuanrong Ji

doi:10.1364/BOE.426366

Centimeter-scale wide-field-of-view laser-scanning photoacoustic microscopy for subcutaneous microvasculature in vivo

Biomed Opt Express. 2021 Apr 28;12(5):2996-3007. doi: 10.1364/BOE.426366. eCollection 2021 May 1.

Authors

Tangyun Liao^{1

2}, Yuan Liu^{1

2}, Junwei Wu^{1

3}, Lijun Deng^{1

4}, Yu Deng³, Lvming Zeng^{1

4}, Xuanrong Ji¹

Affiliations

¹ State Key Laboratory of Precision Electronics Manufacturing Technology and Equipment, Guangdong University of Technology, Guangzhou 510006, China.
² T. Liao and Y. Liu contributed equally to this work.
³ Doppler Electronic Technologies Incorporated Company, Guangzhou 510530, China.
⁴ Key Lab of Optic-Electronic and Communication, Jiangxi Science and Technology Normal University, Nanchang 330038, China.

Abstract

We developed a simple and compact laser-scanning photoacoustic microscopy (PAM) for imaging large areas of subcutaneous microvasculature in vivo. The reflection-mode PAM not only retains the advantage of high scanning speed for optical scanning, but also offers an imaging field-of-view (FOV) up to 20 × 20 mm², which is the largest FOV available in laser-scanning models so far. The lateral resolution of the PAM system was measured to be 17.5 µm. Image experiments on subcutaneous microvasculature in in vivo mouse ears and abdomen demonstrate the system's potential for fast and high-resolution imaging for injuries and diseases of large tissues and organs.