Species Diversity and Antifungal Susceptibilities of Oral Yeasts from Patients with Head and Neck Cancer

Jinyan Wu; Chengwen Gan; Jingyuan Li; Yiwei Liu; Zhongyao Chen; Yunxia Zhang; Guohui Yi; Jinlei Sui; Jianping Xu

doi:10.2147/IDR.S316368

Species Diversity and Antifungal Susceptibilities of Oral Yeasts from Patients with Head and Neck Cancer

Infect Drug Resist. 2021 Jun 18:14:2279-2288. doi: 10.2147/IDR.S316368. eCollection 2021.

Authors

Jinyan Wu^#^{1

2}, Chengwen Gan^#³, Jingyuan Li^{1

2}, Yiwei Liu¹, Zhongyao Chen², Yunxia Zhang^{1

2}, Guohui Yi^{1

2}, Jinlei Sui^{1

2}, Jianping Xu^{2

4}

Affiliations

¹ Key Laboratory of Emergency and Trauma of Ministry of Education, Hainan Medical University, Haikou, 571199, Hainan, People's Republic of China.
² Public Laboratory of Hainan Medical University, Haikou, 571199, Hainan, People's Republic of China.
³ Department of Oral Maxillofacial Surgery, Hainan General Hospital, Haikou, Hainan, 570203, People's Republic of China.
⁴ Department of Biology, McMaster University, Hamilton, Ontario, L8S 4K1, Canada.

^# Contributed equally.

Abstract

Purpose: To investigate the colonization and susceptibility to antifungal drugs of oral yeasts in head and neck cancer patients in Hainan, China.

Methods: Oral mucosa samples from 211 head and neck cancer patients were collected. Oral yeasts were isolated and identified to species by rDNA ITS sequencing. The susceptibilities of all yeasts to amphotericin B, fluconazole, fluorocytosine, itraconazole, and ketoconazole were determined.

Results: Yeasts were isolated from 124 of the 211 oral swabs. The 124 yeast isolates were classified into following 10 species, from the most frequent to the least frequent, Candida albicans (53.2%), Candida tropicalis (22.6%), Candida krusei (6.5%), Kodamaea ohmeri (5.6%), Candida parapsilosis (4.8%), Hanseniaspora opuntiae (2.4%), Candida metapsilosis (1.6%), Pichia terricola (1.6%), Pichia norvegensis (0.8%), and Trichosporon asahii (0.8%). The overall frequencies of resistance among the yeasts to amphotericin B, fluconazole, flucytosine, itraconazole, and ketoconazole were 4.8%, 8.1%, 16.1%, 9.7%, and 9.7%, respectively. One C. albicans strain and one C. tropicalis strain were tolerant/resistant to all five drugs.

Conclusion: Given the high prevalence of oral yeast colonization in head and neck cancer patients and the observed resistance of certain yeast isolates to the five antifungal drugs, our results suggest that rapid identification and susceptibility testing should be implemented before antifungal treatment is applied among patients with head and neck cancer in Hainan.

Keywords: Candida; antifungal resistance; head and neck cancer; oral yeast.

Grants and funding

This project was supported by Natural Science Foundation of Hainan Province (Grant No. 2019RC227), National Natural Science Foundation of China (Grant No. 31860035), and College Student Innovation Project (Grant No. 201911810006).