Lidocaine-loaded nanoparticles are versatile nanomaterials that may be used in pain treatment due to their wound healing properties. The current study describes a wound dressing formulation focused on lidocaine-loaded dextran/ethylene glycol nanoparticles (an anesthetic drug). The lidocaine-loaded dextran/ethylene glycol membranes were fabricated using lidocaine solutions inside the dextran/ethylene glycol medium. The influence of various experimental conditions on dextran/ethylene glycol nanoparticle formations were examined. The sizes of dextran/ethylene glycol and lidocaine-loaded dextran/glycol nanoparticles were examined through the HR-SEM. Moreover, the efficacy antibacterial activity of dextran/glycol and lidocaine-loaded dextran/ethylene glycol nanoparticles was evaluated against the microorganisms grampositive and negative. Furthermore, we observed the In Vivo wound healing of wounds in skin using a mice model over a 16 days period. In this difference to the wounds of untreated mouse, quick healing was observed in the lidocaine-loaded dextran/glycol nanoparticles-treated wounds with fewer injury. These results specify that lidocaine-loaded dextran/ethylene glycol nanoparticles-based dressing material could be a ground-breaking nanomaterial having wound repair and implantations potential required for wound injury in pain management, which was proven using an animal model.