Abstract
Pseudogenes may regulate expression of their parental genes as well as other protein-coding genes through various mechanisms. One such mechanism is the ability to act as competitive endogenous RNA (ceRNA) and participate in microRNA-mediated cross-regulation. Here, we outline how to predict the targets of pseudogene ceRNAs bioinformatically and how to validate them experimentally.
Keywords:
3′UTR; Prediction; Pseudogene; Validation; ceRNA.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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3' Untranslated Regions
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Animals
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Cell Line
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Computational Biology / methods*
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Gene Silencing
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Genomics / methods
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Humans
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MicroRNAs / genetics*
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MicroRNAs / metabolism
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Pseudogenes / genetics*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Small Interfering
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Regulatory Sequences, Ribonucleic Acid / genetics*
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Transfection
Substances
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3' Untranslated Regions
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MicroRNAs
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RNA, Messenger
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RNA, Small Interfering
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Regulatory Sequences, Ribonucleic Acid