Solute-permeable polymer vesicles are structural compartments for nanoreactors/nanofactories in the context of drug delivery and artificial cells. We previously proposed design guidelines for polymers that form solute-permeable vesicles, yet we did not provide enough experimental verification. In addition, the fact that there is no clear factor for identifying permeable solutes necessitates extensive trial and error. Herein, we report solute-permeable polymer vesicles based on an amphiphilic copolymer, thermoresponsive oligosaccharide-block-poly(N-n-propylglycine). The introduction of a thermoresponsive polymer as a hydrophobic segment into amphiphilic polymers is a viable approach to construct solute-permeable polymer vesicles. We also demonstrate that the polymer vesicles are preferentially permeable to cationic and neutral fluorophores and are hardly permeable to anionic fluorophores due to the electrostatic repulsion between the bilayer and anionic fluorophores. In addition, the permeability of neutral fluorophores increases with the increasing log P value of the fluorophores. Thus, the electrical charge and log P value are important factors for membrane permeability. These findings will help researchers develop advanced nanoreactors based on permeable vesicles for a broad range of fundamental and biomedical applications.