Experimental Validation of the Noncoding Potential for lncRNAs

Emily A Dangelmaier; Ashish Lal

doi:10.1007/978-1-0716-1581-2_15

Experimental Validation of the Noncoding Potential for lncRNAs

Methods Mol Biol. 2021:2348:221-230. doi: 10.1007/978-1-0716-1581-2_15.

Authors

Emily A Dangelmaier¹, Ashish Lal²

Affiliations

¹ Regulatory RNAs and Cancer Section, Genetics Branch, Center for Cancer Research (CCR), National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD, USA.
² Regulatory RNAs and Cancer Section, Genetics Branch, Center for Cancer Research (CCR), National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD, USA. ashish.lal@nih.gov.

PMID: 34160810
DOI: 10.1007/978-1-0716-1581-2_15

Abstract

In recent years, long noncoding RNAs (lncRNAs) have been increasingly recognized as critical regulators of a broad spectrum of cellular processes. Recent advancements in proteomic technologies have uncovered that an abundance of noncoding genes, including lncRNAs, have been misannotated and in reality encode proteins. This revelation underscores the need to accurately determine the coding potential of lncRNAs prior to assessment of their functional mechanisms. Here, we detail numerous experimental techniques useful in the determination of lncRNA coding potential. Several of these methods are doubly useful in that they may also be employed in studying the function of a lncRNA, be it via an RNA, protein, or both.

Keywords: Coding potential; Long noncoding RNAs; Micropeptides; Translation; lncRNAs.

Publication types

Research Support, N.I.H., Intramural
Review

MeSH terms

Animals
CRISPR-Cas Systems
Computational Biology / methods
Gene Expression Regulation*
Humans
Molecular Sequence Annotation
Open Reading Frames
Polyribosomes / metabolism
Protein Biosynthesis*
RNA, Long Noncoding / genetics*

Substances

RNA, Long Noncoding