Complete mitochondrial genomes of two marine monogonont rotifer Brachionus manjavacas strains

Min-Sub Kim; Young Hwan Lee; Duck-Hyun Kim; Hee-Jin Kim; Un-Ki Hwang; Russell Shiel; Atsushi Hagiwara; Jae-Seong Lee

doi:10.1080/23802359.2021.1935349

Complete mitochondrial genomes of two marine monogonont rotifer Brachionus manjavacas strains

Mitochondrial DNA B Resour. 2021 Jun 7;6(7):1921-1923. doi: 10.1080/23802359.2021.1935349.

Authors

Min-Sub Kim¹, Young Hwan Lee¹, Duck-Hyun Kim¹, Hee-Jin Kim², Un-Ki Hwang³, Russell Shiel⁴, Atsushi Hagiwara^{2

5}, Jae-Seong Lee¹

Affiliations

¹ Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon, South Korea.
² Institute of Integrated Science and Technology, Nagasaki University, Nagasaki, Japan.
³ Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Institute of Fisheries Science, Incheon, South Korea.
⁴ School of Biological Sciences, University of Adelaide, Adelaide, Australia.
⁵ Organization for Marine Science and Technology, Nagasaki University, Nagasaki, Japan.

Abstract

The complete mitochondrial genomes of Brachionus manjavacas German strain were 10,721 bp (mitochondrial DNA I) and 12,274 bp (mitochondrial DNA II) in size, while the complete mitochondrial genomes of B. manjavacas Australian strain were 10,889 bp (mitochondrial DNA I) and 12,443 bp (mitochondrial DNA II) in size. Of 12 protein-coding genes (PCGs), 99.6% of amino acid sequences were identical between the two strains. Of 12 PCGs of both B. manjavacas strains, three genes (ND1, ATP6, and ND5) had incomplete stop codon T. Furthermore, ATA was the start codon for ND4, ND5, and CO3 genes, whereas that for other PCGs was ATG in both strains. The base compositions of 12 PCGs in the B. manjavacas strains were similar, indicating that the mitochondrial genome of the two strains was structurally conserved over evolution. The gene structure and its orientation of 12 PGCs of B. manjavacas strains were identical, as shown in other marine Brachionus rotifers and the freshwater Brachionus rotifers, while the freshwater rotifer B. calyciflorus had an additional cytochrome b gene in the mitochondrial DNA I.

Keywords: Australian strain; Brachionus manjavacas; German strain; Monogonont rotifer; complete mitochondrial genome.

Grants and funding

This research was supported by the Collaborative Genome Program of the Korea Institute of Marine Science and Technology Promotion funded by the Ministry of Oceans and Fisheries [No. 20180430] and also supported by a grant from the National Institute of Fisheries Science, Korea [R2021033].