A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants

Gongyao Shi; Mengyuan Hao; Baoming Tian; Gangqiang Cao; Fang Wei; Zhengqing Xie

doi:10.3389/fpls.2021.671091

A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants

Front Plant Sci. 2021 Jun 4:12:671091. doi: 10.3389/fpls.2021.671091. eCollection 2021.

Authors

Gongyao Shi^{1

2}, Mengyuan Hao^{1

2}, Baoming Tian^{1

3}, Gangqiang Cao^{1

3}, Fang Wei^{1

3}, Zhengqing Xie^{1

3}

Affiliations

¹ Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, China.
² School of Life Sciences, Zhengzhou University, Zhengzhou, China.
³ Henan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, China.

Abstract

As a promising high-throughput reverse genetic tool in plants, virus-induced gene silencing (VIGS) has already begun to fulfill some of this promise in diverse aspects. However, review of the technological advancements about widely used VIGS system, tobacco rattle virus (TRV)-mediated gene silencing, needs timely updates. Hence, this article mainly reviews viral vector construction, inoculation method advances, important influential factors, and summarizes the recent applications in diverse plant species, thus providing a better understanding and advice for functional gene analysis related to crop improvements.

Keywords: TRV-VIGS; agroinfiltration; methodology modification; secondary inoculation; vector construction.

Publication types

Review

Associated data

figshare/10.6084/m9.figshare.14626272