The advancements, challenges, and future implications of the CRISPR/Cas9 system in swine research

Jinfu Zhang; Emmanuel M Khazalwa; Hussein M Abkallo; Yuan Zhou; Xiongwei Nie; Jinxue Ruan; Changzhi Zhao; Jieru Wang; Jing Xu; Xinyun Li; Shuhong Zhao; Erwei Zuo; Lucilla Steinaa; Shengsong Xie

doi:10.1016/j.jgg.2021.03.015

The advancements, challenges, and future implications of the CRISPR/Cas9 system in swine research

J Genet Genomics. 2021 May 20;48(5):347-360. doi: 10.1016/j.jgg.2021.03.015. Epub 2021 May 11.

Authors

Affiliations

¹ Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, PR China.
² Animal and Human Health Program, Biosciences, International Livestock Research Institute (ILRI), P.O. Box 30709, Nairobi 00100, Kenya.
³ Key Laboratory of Pig Molecular Quantitative Genetics of Anhui Academy of Agricultural Sciences, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety Engineering, Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, PR China.
⁴ Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, PR China; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, PR China.
⁵ Lingnan Guangdong Laboratory of Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, PR China. Electronic address: erweizuo@163.com.
⁶ Animal and Human Health Program, Biosciences, International Livestock Research Institute (ILRI), P.O. Box 30709, Nairobi 00100, Kenya. Electronic address: l.steinaa@cgiar.org.
⁷ Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, PR China; Animal and Human Health Program, Biosciences, International Livestock Research Institute (ILRI), P.O. Box 30709, Nairobi 00100, Kenya; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address: ssxie@mail.hzau.edu.cn.

PMID: 34144928
DOI: 10.1016/j.jgg.2021.03.015

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) genome editing technology has dramatically influenced swine research by enabling the production of high-quality disease-resistant pig breeds, thus improving yields. In addition, CRISPR/Cas9 has been used extensively in pigs as one of the tools in biomedical research. In this review, we present the advancements of the CRISPR/Cas9 system in swine research, such as animal breeding, vaccine development, xenotransplantation, and disease modeling. We also highlight the current challenges and some potential applications of the CRISPR/Cas9 technologies.

Keywords: Base editing; CRISPR screening; CRISPR/Cas9; Genome editing; Swine.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Animals, Genetically Modified
Breeding
CRISPR-Cas Systems*
Disease Resistance
Gene Editing / methods*
Gene Editing / trends*
Genetic Association Studies / methods
Genetic Association Studies / trends
Genetic Engineering / methods
Genetic Engineering / trends
Humans
Models, Animal
Quantitative Trait Loci
Quantitative Trait, Heritable
Research / trends*
Swine