Plug and play with recombinant antibody fragments

Ashley K Grahn; Christina J Miller; Brian K Kay

doi:10.1016/j.chembiol.2021.05.017

Plug and play with recombinant antibody fragments

Cell Chem Biol. 2021 Jun 17;28(6):745-747. doi: 10.1016/j.chembiol.2021.05.017.

Authors

Ashley K Grahn¹, Christina J Miller¹, Brian K Kay²

Affiliations

¹ Tango Biosciences, Chicago, IL 60612, USA.
² Tango Biosciences, Chicago, IL 60612, USA; University of Illinois at Chicago, Chicago, IL 60607, USA. Electronic address: bkay@uic.edu.

PMID: 34143957
DOI: 10.1016/j.chembiol.2021.05.017

Abstract

In this issue of Cell Chemical Biology, Hentrich et al. (2021) describe the application of the SpyCatcher technology to antibody discovery and validation. Fab-SpyTag fusion proteins can be expressed in the periplasm of protease-deficient bacteria and coupled in a modular manner to a variety of SpyCatcher-tagged proteins for improved assay performance.

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MeSH terms

Immunoglobulin Fragments*
Recombinant Proteins / genetics

Substances

Immunoglobulin Fragments
Recombinant Proteins