iTRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlings

Jing Wang; Chengliang Liang; Sha Yang; Jingshuang Song; Xuefeng Li; Xiongze Dai; Fei Wang; Niran Juntawong; Fangjun Tan; Xilu Zhang; Chunhai Jiao; Xuexiao Zou; Wenchao Chen

doi:10.7717/peerj.11509

iTRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlings

PeerJ. 2021 Jun 3:9:e11509. doi: 10.7717/peerj.11509. eCollection 2021.

Authors

Jing Wang^#^{1

2}, Chengliang Liang^#¹, Sha Yang¹, Jingshuang Song¹, Xuefeng Li¹, Xiongze Dai³, Fei Wang⁴, Niran Juntawong⁵, Fangjun Tan¹, Xilu Zhang¹, Chunhai Jiao⁴, Xuexiao Zou³, Wenchao Chen¹

Affiliations

¹ Vegetable Research Institute, Hunan Academy of Agricultural Sciences, Changsha, China.
² Longping Branch, Graduate School of Hunan University, Changsha, China.
³ College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China.
⁴ Hubei Academy of Agricultural Sciences, Wuhan, China.
⁵ Faculty of Science, Department of Botany, Kasetsart University, Bangkok, Thailand.

^# Contributed equally.

Abstract

Background: As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper.

Methods: To understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress.

Result: In the present study, we investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper cultivars in response to heat stress. Phenotypic and physiological changes showed that 17CL30 had a stronger ability to resist heat stress compared with 05S180. In proteomic analysis, a total of 3,874 proteins were identified, and 1,591 proteins were considered to participate in the process of heat stress response. According to bioinformatic analysis of heat-responsive proteins, the heat tolerance of 17CL30 might be related to a higher ROS scavenging, photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180.

Keywords: Heat stress; Pepper; Physiological; Proteomics.

Grants and funding

This work was supported by the National Key R&D Program of China (2016YFE0205500), the key research and development program of Hunan (2019NK2191) and the local standards of Hunan (DB43/T 1702-2019). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.