Molecular detection of Cryptosporidium: an emerging parasite in different water sources of 2010 flood-affected district Nowshera, Pakistan

Arch Microbiol. 2021 Sep;203(7):4397-4403. doi: 10.1007/s00203-021-02419-y. Epub 2021 Jun 13.

Abstract

Cryptosporidium is a water-borne zoonotic parasite worldwide, usually found in lakes and rivers contaminated with sewage and animal wastes, causing outbreaks of cryptosporidiosis. In this study, 300 water samples were collected from four designated places of flood-affected district Nowshera consist of different water sources to find out the prevalence of Cryptosporidium via polymerase chain reaction (PCR). The overall prevalence of Cryptosporidium was 30.33% (91/300) with more prevalent 44% in drain water and low 5% in bore/tube well water. The prevalence in open well and tap water was recorded 33% and 20%, respectively. The highest prevalence was recorded in summer (June-September). The result of this study ensures enormous contamination of drinking water that requires appropriate treatment, cleaning and filtration to provide safe drinking water. Preventing water-borne disease and proper treatment of water supplies is essential to public health.

Keywords: Cryptosporidium; Diarrhea; PCR; Zoonotic parasite.

MeSH terms

  • Cryptosporidium* / genetics
  • Environmental Monitoring*
  • Floods*
  • Pakistan
  • Water* / parasitology

Substances

  • Water