Changes in the structure and digestibility of myoglobin treated with sodium chloride

Hui Liu; Qian Li; Di Zhao; Miao Zhang; Shuai Jiang; Chunbao Li

doi:10.1016/j.foodchem.2021.130284

Changes in the structure and digestibility of myoglobin treated with sodium chloride

Food Chem. 2021 Nov 30:363:130284. doi: 10.1016/j.foodchem.2021.130284. Epub 2021 Jun 3.

Authors

Hui Liu¹, Qian Li¹, Di Zhao¹, Miao Zhang¹, Shuai Jiang¹, Chunbao Li²

Affiliations

¹ Key Laboratory of Meat Processing and Quality Control, MOE, Key Laboratory of Meat Processing, MARA, Jiangsu Collaborative Innovation Center of Meat Production, Processing and Quality Control; College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
² Key Laboratory of Meat Processing and Quality Control, MOE, Key Laboratory of Meat Processing, MARA, Jiangsu Collaborative Innovation Center of Meat Production, Processing and Quality Control; College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: chunbao.li@njau.edu.cn.

PMID: 34120050
DOI: 10.1016/j.foodchem.2021.130284

Abstract

Myoglobin is a protein not easily broken down by digestive enzymes due to its rigid structure. This study evaluated the structural characteristics of myoglobin under various sodium chloride treatments (0.4-0.8 mol/L for 5-10 h) and the impacts on its digestibility using spectroscopic and molecular dynamics simulation techniques. Myoglobin digestibility was 40% following pepsin digestion and 60% after being sequentially digested by pepsin and trypsin. The α-helix content of myoglobin did not change significantly following sodium chloride treatment but hydrophobic amino acids were exposed and the binding of phenylalanine targeted by some digestive enzymes became more stable, leading to the reduced digestibility.

Keywords: Digestibility; Molecular dynamics simulation; Myoglobin; Sodium chloride; Structure.

MeSH terms

Amino Acids
Myoglobin / chemistry*
Pepsin A
Sodium Chloride*

Substances

Amino Acids
Myoglobin
Sodium Chloride
Pepsin A