Carbapenemases producing Klebsiella pneumoniae from the pus of hospitalized patients: In-vitro antibiotic properties of Streptomyces against multidrug resistant infectious bacteria

Balamuralikrishnan Balasubramanian; Natarajan Benit; Paul Agastian; Khalid S Almaary; Turki M Dawoud; Yahya B Elbadawi; Ayman Mubarak; Mohammed S Alfadul; Reem M Aljowaie

doi:10.1016/j.jiph.2021.05.006

Carbapenemases producing Klebsiella pneumoniae from the pus of hospitalized patients: In-vitro antibiotic properties of Streptomyces against multidrug resistant infectious bacteria

J Infect Public Health. 2021 Jul;14(7):892-897. doi: 10.1016/j.jiph.2021.05.006. Epub 2021 May 27.

Authors

Balamuralikrishnan Balasubramanian¹, Natarajan Benit², Paul Agastian³, Khalid S Almaary⁴, Turki M Dawoud⁵, Yahya B Elbadawi⁵, Ayman Mubarak⁵, Mohammed S Alfadul⁵, Reem M Aljowaie⁵

Affiliations

¹ Department of Food Science and Biotechnology, College of Life Science, Sejong University, Seoul 05006, South Korea.
² Department of Botany, Holy Cross College, ManonmaniamSundaranar University, Abishekapatti, Tirunelveli 627 012, Tamil Nadu, India.
³ Research Department of Plant Biology and Biotechnology, Loyola College, Chennai, Tamil Nadu, India.
⁴ Department of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia. Electronic address: kalmaary@ksu.edu.sa.
⁵ Department of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia.

PMID: 34119841
DOI: 10.1016/j.jiph.2021.05.006

Abstract

Background: Klebsiella pneumoniae is predominantly exists in the pus of the human wounds and it creates massive infections in the skin and causes serious health associated infections. Modern antibiotics are highly active in the treatment of wound infections. In this study was aimed to determine resistance of K. pneumoniae screened from wound specimens of patients. Sample was collected from the pus of the patients associated with secondary infection.

Methods: Samples were serially diluted and the isolated bacterial strains were characterized by biochemical tests, colony morphology and Gram's staining methods. Resistance of K. pneumoniae was tested using antibiotics such as, Gentamycin, Ampicillin, Tetracycline, Cefurooxime, Oxacillin, Ofloxacin, Erythromycin, Nalidic acid, Cefepine, Piperacillin, Norfloxacin, Imipenem, Nitrofurantoin, Amikacin, Ciprofloxacin, Vancomycin, Meropeneum and Cefotaxime with Kirby-Bauer disc diffusion method.

Results: Among the 73 K. pneumoniae strains, four strains produced AmpC and ESBLs, 42 strains produced ESBLs and 7 bacterial strains synthesized only AmpC enzyme. Four stains produced ESBLs and showed multidrug resistance against various antibiotics. Most of the strains synthesized extracellular polysaccharides and mediated biofilm formation. Among the K. pneumoniae strains, K. pneumoniae PS02 showed multidrug resistant against most of the tested antibiotics. It produced ESBLs and AmpC enzyme. To produce secondary metabolites, actinomycetes were isolated and characterized as Streptomyces sp. AC14. The secondary metabolite was effective against Klebsiella strains.

Conclusions: To conclude, secondary metabolites extracted from Streptomyces sp. AC14 was found to be effective against multidrug resistant bacterium. Further studies are warranted to analyze the drug hydrolyzing pathways of bacteria and to identify the mechanism of action of secondary metabolites from Streptomyces sp. AC14.

Keywords: Actinomycetes; Antibiotics; Drug resistance bacteria; Klebsiella pneumoniae; Wound Infection.

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Proteins
Humans
Klebsiella Infections* / drug therapy
Klebsiella pneumoniae
Microbial Sensitivity Tests
Streptomyces*
Suppuration
beta-Lactamases

Substances

Anti-Bacterial Agents
Bacterial Proteins
beta-Lactamases
carbapenemase