Functional characterization of the transcription factors AhR and ARNT in Nilaparvata lugens

Yue Wang; Ruoheng Jin; Chaoya Liu; Yuanyuan Gao; Xiaoqian Deng; Hu Wan; Jianhong Li

doi:10.1016/j.pestbp.2021.104875

Functional characterization of the transcription factors AhR and ARNT in Nilaparvata lugens

Pestic Biochem Physiol. 2021 Jul:176:104875. doi: 10.1016/j.pestbp.2021.104875. Epub 2021 May 14.

Authors

Yue Wang¹, Ruoheng Jin¹, Chaoya Liu¹, Yuanyuan Gao¹, Xiaoqian Deng¹, Hu Wan¹, Jianhong Li²

Affiliations

¹ Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
² Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address: jianhl@mail.hzau.edu.cn.

PMID: 34119220
DOI: 10.1016/j.pestbp.2021.104875

Abstract

In the present study, the aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) of Nilaparvata lugens were cloned and identified. The NlAhR and NlARNT expression levels significantly increased after imidacloprid, etofenprox and isoprocarb treatments. Knockdowns of NlAhR and NlARNT increased the susceptibility of N. lugens to imidacloprid, etofenprox and isoprocarb, and the detoxification enzyme activities were also significantly decreased. In addition, NlCYP301A1, NlGSTt1 and NlCarE7 were significantly down-regulated after injections of dsNlAhR and dsNlARNT, with the NlCarE7 expression decreasing by greater than 80%. Moreover, after knocking down NlCarE7, the susceptibility of N. lugens to etofenprox and isoprocarb significantly increased. Both NlAhR and NlARNT bound the NlCarE7 promoter and significantly enhanced the transcriptional activity. Our research revealed the functional roles of transcription factors NlAhR and NlARNT in the detoxification metabolism of N. lugens. The results provide a theoretical basis for the pest management and comprehensive control of N. lugens and increase our knowledge of insect toxicology.

Keywords: ARNT; AhR; Brown planthopper; Detoxification metabolism; Transcriptional regulation.

MeSH terms

Animals
Aryl Hydrocarbon Receptor Nuclear Translocator* / genetics
Hemiptera* / genetics
Hemiptera* / metabolism
Insecticide Resistance / genetics
Receptors, Aryl Hydrocarbon* / genetics
Receptors, Aryl Hydrocarbon* / metabolism

Substances

Receptors, Aryl Hydrocarbon
Aryl Hydrocarbon Receptor Nuclear Translocator