New concept in swine wastewater treatment: development of a self-sustaining synergetic microalgae-bacteria symbiosis (ABS) system to achieve environmental sustainability

Abstract

Much attention has been paid to developing methods capable of synchronous removal of pollutants from swine wastewater. Due to the natural symbiotic interactions between microalgae and bacteria, the microalgae-bacteria symbiosis (ABS) system has been found to have potential for treating wastewater. However, the corresponding biological mechanisms in the ABS system and the role of dynamic microbial community evolution in pollutant removal systems remain poorly understood. Therefore, we investigate the potential of an ABS system for pollutant removal applications and analyze the bacterial consortium symbiotically combined with Chlorella sp. MA1 and Coelastrella sp. KE4. The NH₄⁺-N and PO₄^3--P removal efficiencies were significantly increased from 12.79% to 99.52% and 35.66% to 96.06% due to biotic interactions between the microalgae and bacteria. The abundance of bacterial taxa and genes related to oxidative stress, cell growth and nitrogen transfer were found to increase in response to photosynthesis, respiration and NH₄⁺-N uptake. Furthermore, pathogen inactivation was induced via microalgae, co-driven by microbial succession under high dissolved oxygen conditions. In this microalgae-enhanced ABS system, the interactions between microalgae and bacteria are established for pathogens elimination and nitrogen cycling, verifying that the ABS system is an effective and environmentally sustainable swine wastewater treatment method.

Keywords: Bacterial community; Environmental sustainability; Microalgae-bacteria symbiosis (ABS); Swine wastewater treatment; Synergistic interaction.