Characterization of antifungal C-type lectin receptor expression on murine epithelial and endothelial cells in mucosal tissues

Eur J Immunol. 2021 Sep;51(9):2341-2344. doi: 10.1002/eji.202149192. Epub 2021 Jun 23.

Abstract

Our data reveal that selection of enzymes for generating single cell suspensions from murine tissues influences detection of surface expression of antifungal CLRs. Using a method that most preserves receptor expression, we show that non-myeloid expression of antifungal CLRs is limited to MelLec on endothelial cells in murine mucosal tissues.

Keywords: C-type lectin; Endothelial cells; Epithelial cells; Immunity, Innate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aspergillus / immunology
  • Candida / immunology
  • Cryptococcus / immunology
  • Endothelial Cells / metabolism*
  • Epithelial Cells / metabolism*
  • Fungi / immunology*
  • Lectins, C-Type / metabolism*
  • Mice
  • Mucous Membrane / immunology*
  • Mucous Membrane / metabolism
  • Mucous Membrane / microbiology

Substances

  • Lectins, C-Type