Our data reveal that selection of enzymes for generating single cell suspensions from murine tissues influences detection of surface expression of antifungal CLRs. Using a method that most preserves receptor expression, we show that non-myeloid expression of antifungal CLRs is limited to MelLec on endothelial cells in murine mucosal tissues.
Keywords: C-type lectin; Endothelial cells; Epithelial cells; Immunity, Innate.
© 2021 The Authors. European Journal of Immunology published by Wiley-VCH GmbH.