On the one hand, lensless imaging technology has become one of the key technologies to achieve point-of-care testing; on the other hand, microfluidic technology has shown great application potential in the field of biological detection. Using mainstream lensless imaging technology to achieve biological cell imaging in microfluidic chips has technical limitations. In particular, it is more difficult to achieve lensless imaging for non-spherical cells in microfluidic chips such as red blood cells. Achieving red blood cell recognition and posture estimation in a microfluidic chip under the lensless imaging, combined with mainstream lensless imaging technology, can provide more effective red blood cell morphological parameters for medical diagnosis. In this paper, the method for red blood cell recognition and posture estimation in microfluidic chips based on lensless imaging is given. First, the relevant theoretical basis is introduced. Then, the models of red blood cell recognition and posture estimation in microfluidic chips based on lensless imaging are given. The effect of red blood cell flipping on lensless imaging is analyzed in the modeling process. Finally, the effectiveness of the proposed method is verified by experiments. Experiments show that the proposed method can well achieve red blood cell recognition and posture estimation through the shape characteristics of red blood cells.
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