Prostaglandin E2 promotes Staphylococcus aureus infection via EP4 receptor in bovine endometrium

Kun Liu; Wei Mao; Bo Liu; Tingting Li; Xinfei Wang; Le Pei; Jinshan Cao; Fenglong Wang

doi:10.1016/j.micpath.2021.105019

Prostaglandin E2 promotes Staphylococcus aureus infection via EP4 receptor in bovine endometrium

Microb Pathog. 2021 Sep:158:105019. doi: 10.1016/j.micpath.2021.105019. Epub 2021 Jun 6.

Authors

Kun Liu¹, Wei Mao², Bo Liu³, Tingting Li⁴, Xinfei Wang⁵, Le Pei⁶, Jinshan Cao⁷, Fenglong Wang⁸

Affiliations

¹ Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, 010018, Hohhot, China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Anima Disease, Ministry of Agriculture, Hohhot, China; Laboratory of Veterinary Pathology, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China. Electronic address: liukun8066340@163.com.
² Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, 010018, Hohhot, China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Anima Disease, Ministry of Agriculture, Hohhot, China. Electronic address: maowei820115@163.com.
³ Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, 010018, Hohhot, China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Anima Disease, Ministry of Agriculture, Hohhot, China. Electronic address: liubo8510@imau.edu.cn.
⁴ Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, 010018, Hohhot, China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Anima Disease, Ministry of Agriculture, Hohhot, China. Electronic address: litingting0505@sina.cn.
⁵ Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, 010018, Hohhot, China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Anima Disease, Ministry of Agriculture, Hohhot, China. Electronic address: W15828164878@163.com.
⁶ Veterinary Research Institute, Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Hohhot, China. Electronic address: peihappypl@163.com.
⁷ Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, 010018, Hohhot, China; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Anima Disease, Ministry of Agriculture, Hohhot, China. Electronic address: Jinshancao@imau.edu.cn.
⁸ Laboratory of Veterinary Pathology, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China. Electronic address: wangfengloo@imau.edu.cn.

PMID: 34107344
DOI: 10.1016/j.micpath.2021.105019

Abstract

Prostaglandin E2 (PGE₂) enhances Staphylococcus aureus infection but its mechanism is not well understood. Here, we examined the effect of PGE₂ on Staphylococcal Protein A (SPA) expression in bovine endometrium and determined the role of select PGE₂ receptors (i.e., EP2 and EP4) in adhesion and internalization of S. aureus. S. aureus isolate SA113 was used for in vitro infection of bovine endometrial tissues and epithelial cells, with treatment conditions consisting of untreated control, SA113 treatment, SA113 + PGE₂, SA113 + PGE₂ + EP2 receptor antagonist (AH-6809), and SA113 + PGE₂ + EP4 receptor antagonist (AH-23848). Immunofluorescence assay revealed that PGE₂ could promote SPA expression in S. aureus-infected bovine endometrial tissues. PGE₂ also enhanced the adhesion and internalization of S. aureus in bovine endometrial cells. The addition of EP4 antagonist, but not the EP2 antagonist, abrogated the ability of PGE₂ to promote S. aureus SPA expression, adhesion, and internalization in endometrial cells. Our findings suggest that S. aureus infection in the endometrium is enhanced by PGE₂ through the EP4 receptor. This result is essential for the development of new approach to treating S. aureus infection, such as the application of EP4 antagonist as an adjunct drug treatment.

Keywords: Bovine endometritis; EP4 receptor; Infection; Prostaglandin E2; S. aureus.

MeSH terms

Animals
Cattle
Dinoprostone*
Endometrium
Female
Receptors, Prostaglandin E, EP2 Subtype
Staphylococcal Infections* / veterinary
Staphylococcus aureus

Substances

Receptors, Prostaglandin E, EP2 Subtype
Dinoprostone