Objective: To investigate the therapeutic effect of spleen low molecular weight extracts on epileptics hydrochloride-induced leukopenia in mice and explore its mechanism.
Methods: The model of leukopenia in mice was established by the injection of epirubicin hydrochloride (10 mg/kg). After the injection of chemotherapeutic drugs, leukocytopenia mice were treated with different doses of spleen low molecular weight extract, Ganoderma oral solution and recombinant granulocyte colony stimulating factor (rhG-CSF). The general survival status indicators such as body weight, coat color and athletic ability of mice in each group were recorded; the tail vein blood of mice in each group was collected and the white blood cell count in them was calculated; bone marrow of mice was taken and bone marrow smears were observed.
Results: In the model group, the weight of the mice gradually decreased in the later period, their coat became dark and rough, and the ability to exercise decreased, while the mice in the treatment groups showed different degrees of improvement in their survival status except for the mice treated by rhG-CSF. There was no significant fluctuation in the white blood cell count of the blank control mice. After injection of epirubicin, the white blood cell count of peripheral blood in the model mice and treated mice were decreased. The white blood cell count was lower in the mice treated with high-dose low molecular weight extract and rhG-CSF than that in other experimental groups. Bone marrow smear showed that the proportion of bone marrow nucleated cells in the mice treated with the low molecular weight extract of the spleen was significantly higher than that of model mice (P<0.05).
Conclusion: The low molecular weight spleen extracts can significantly improve the hematopoietic state of mouse bone marrow, promote the proliferation of inhibited bone marrow cells, and thus has the effect of treating leukopenia in mice.
题目: 脾低分子量提取物对小鼠白细胞减少症治疗作用及其机制的研究.
目的: 研究脾低分子量提取物对盐酸表柔比星引起的小鼠白细胞减少症的治疗效果并探讨其相关机制.
方法: 利用注射盐酸表柔比星（10 mg/kg）的方法，建立小鼠白细胞减少症的模型。在注射化疗药物之后，分别以不同剂量的脾低分子量提取物、灵芝口服液及重组粒细胞集落刺激因子（rhG-CSF）对白细胞减少症小鼠进行治疗。观察并记录各组小鼠体重、毛色及运动能力等一般生存状态指标；取各组小鼠尾静脉血并计数其中白细胞数量；取小鼠骨髓，观察骨髓涂片.
结果: 模型组小鼠体重后期逐渐下降，且出现毛色变暗、毛质粗糙、运动能力下降等现象；治疗组除应用rhG-CSF的动物以外，生存状况均有不同程度的改善。空白对照组小鼠白细胞数无明显波动；模型组及各治疗组小鼠注射表柔比星之后，外周血白细胞数均有所下降；高剂量脾低分子量提取物及rhG-CSF注射液治疗组小鼠白细胞数下降趋势低于其他各实验组。骨髓涂片结果显示，脾低分子量提取物治疗组小鼠骨髓象有核细胞占比显著高于模型组小鼠（P<0.05）.
结论: 脾低分子量提取物能够显著改善小鼠骨髓造血状态，促进受抑制的骨髓细胞增生，从而具有治疗小鼠白细胞减少症的作用.