Mitochondrial DNA sequence of the horseshoe crab Tachypleus gigas

Maria E Sarmiento; Kai Ling Chin; Nyok Sean Lau; Ismail Aziah; Mohd Nor Norazmi; Armando Acosta; Noraznawati Ismail; Nik Soriani Yaacob

doi:10.1080/23802359.2021.1930213

Mitochondrial DNA sequence of the horseshoe crab Tachypleus gigas

Mitochondrial DNA B Resour. 2021 May 23;6(6):1710-1714. doi: 10.1080/23802359.2021.1930213.

Authors

Maria E Sarmiento¹, Kai Ling Chin², Nyok Sean Lau³, Ismail Aziah⁴, Mohd Nor Norazmi¹, Armando Acosta¹, Noraznawati Ismail⁵, Nik Soriani Yaacob⁶

Affiliations

¹ School of Health Sciences, Universiti Sains Malaysia, Health Campus, Kelantan, Malaysia.
² Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia.
³ Centre for Chemical Biology, Universiti Sains Malaysia, Pulau Pinang, Malaysia.
⁴ Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Health Campus, Kelantan, Malaysia.
⁵ Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Terengganu, Malaysia.
⁶ Department of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, Kelantan, Malaysia.

Abstract

This paper reports on the complete mitochondrial (mt) genome of a horseshoe crab, Tachypleus gigas (T. gigas), in Kuala Kemaman, Terengganu, Malaysia. Whole-genome sequencing of hemocyte DNA was performed with Illumina HiSeq system and the generated reads were de novo assembled with ABySS 2.1.5 and reassembled using mitoZ against Carcinoscorpius rotundicauda and Limulus polyphemus, resulting in a contig of 15 Kb. Phylogenetic analysis of the assembled mt genome suggests that the Tachypleus gigas is closely related to Tachypleus tridentatus than to Carcinoscorpius rotundicauda.

Keywords: Malaysia; Tachypleus gigas; Terengganu; mitochondrial genome; phylogenetic analysis; whole genome sequencing.

Grants and funding

This work was supported by the Universiti Sains Malaysia [304/PPSP/602002].