Detection of bladder cancer with aberrantly fucosylated ITGA3

Anal Biochem. 2021 Sep 1:628:114283. doi: 10.1016/j.ab.2021.114283. Epub 2021 Jun 5.

Abstract

We describe a simple, non-invasive assay to identify fucosylated-glycoisoform of integrin alpha-3 (ITGA3) directly from unprocessed urine. ITGA3 was detected directly from the urine of bladder cancer (BlCa) (n = 13) and benign prostatic hyperplasia (BPH) (n = 9) patients with the use of lectins coated on europium-doped-nanoparticles (Eu3+-NPs). Lectin Ulex europaeus agglutinin-I (UEA) showed enhanced binding with BlCa-derived ITGA3. The evaluation with individual samples showed that a glycovariant ITGA3-UEA assay could significantly discriminate BlCa from BPH patients (p = 0.007). The detection of aberrantly fucosylated-isoform of ITGA3 from urine can be used to distinguish BlCa from age-matched benign controls in a simple sandwich assay.

Keywords: Bladder cancer; Glycosylations; ITGA3; Integrin; UEA-lectin; Urine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / metabolism
  • Biomarkers, Tumor / urine*
  • Female
  • Fucose / metabolism
  • Humans
  • Integrin alpha3 / metabolism
  • Integrin alpha3 / urine*
  • Male
  • Middle Aged
  • Urinary Bladder Neoplasms / diagnosis*
  • Urinary Bladder Neoplasms / metabolism
  • Urinary Bladder Neoplasms / urine

Substances

  • Biomarkers, Tumor
  • ITGA3 protein, human
  • Integrin alpha3
  • Fucose