Natural Molybdenite- and Tyrosinase-Based Amperometric Catechol Biosensor Using Acridine Orange as a Glue, Anchor, and Stabilizer for the Adsorbed Tyrosinase

Yan Zhang; Yue Wang; Zhiqiang Zhang; Ahmed Sobhy; Susumu Sato; Masaya Uchida; Yasushi Hasebe

doi:10.1021/acsomega.1c00973

Natural Molybdenite- and Tyrosinase-Based Amperometric Catechol Biosensor Using Acridine Orange as a Glue, Anchor, and Stabilizer for the Adsorbed Tyrosinase

ACS Omega. 2021 May 18;6(21):13719-13727. doi: 10.1021/acsomega.1c00973. eCollection 2021 Jun 1.

Authors

Yan Zhang¹, Yue Wang¹, Zhiqiang Zhang¹, Ahmed Sobhy^{2

3}, Susumu Sato⁴, Masaya Uchida⁵, Yasushi Hasebe⁶

Affiliations

¹ School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Middle Road, High-tech Zone, Anshan, Liaoning 114051, China.
² School of Resources and Environmental Engineering, Shandong University of Technology, Zibo, Shandong 255049, China.
³ Central Metallurgical Research and Development Institute, Helwan, Cairo 11421, Egypt.
⁴ Department of Information Systems, Saitama Institute of Technology, 1690 Fusaiji, Fukaya, Saitama 369-0293, Japan.
⁵ Advanced Science Research Laboratory, Saitama Institute of Technology, 1690 Fusaiji, Fukaya, Saitama 369-0293, Japan.
⁶ Department of Life Science and Green Chemistry, Saitama Institute of Technology, 1690 Fusaiji, Fukaya, Saitama 369-0293, Japan.

Abstract

To develop a natural mineral-based electrochemical enzyme biosensor, natural molybdenite (MLN), tyrosinase (TYR), and acridine orange (AO) were coadsorbed onto a glassy carbon electrode (GCE). The developed TYR/AO/MLN-GCE-based amperometric TYR biosensor exhibited excellent performance for highly sensitive determination of catechol (linear range, 0.1-80 μM; sensitivity, 0.0315 μA/μM; LOD, 0.029 μM; response time, <4 s) with good reproducibility and good operational and storage stabilities. The electrochemical impedance spectroscopy (EIS) and quartz crystal microbalance with dissipation (QCM-D) revealed interesting roles of AO: (1) an efficient glue for enhancing the amount of the adsorbed TYR on the MLN-GCE, (2) an anchor for efficient orientation of the adsorbed TYR on the MLN-GCE, and (3) a stabilizer providing a suitable microenvironment for the adsorbed TYR on the MLN-GCE surface. This physical adsorption-based AO-coupled enzyme-modification strategy onto natural MLN would be a versatile strategy to develop cost-effective and environment-friendly natural mineral-based electrochemical biosensors and bioelectronic devices.