The role of LncRNA MALAT-1 and MiRNA-9 in Psoriasis

Azza M Elamir; Olfat G Shaker; Mohamed Hm El-Komy; Mai Mahmoud Sharabi; Nesreen M Aboraia

doi:10.1016/j.bbrep.2021.101030

The role of LncRNA MALAT-1 and MiRNA-9 in Psoriasis

Biochem Biophys Rep. 2021 May 25:26:101030. doi: 10.1016/j.bbrep.2021.101030. eCollection 2021 Jul.

Authors

Azza M Elamir¹, Olfat G Shaker², Mohamed Hm El-Komy³, Mai Mahmoud Sharabi¹, Nesreen M Aboraia⁴

Affiliations

¹ Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Fayoum University, Fayoum, Egypt.
² Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
³ Dermatology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
⁴ Dermatology Department, Faculty of Medicine, Fayoum University, Fayoum, Egypt.

Abstract

Background: Psoriasis is a chronic skin disorder manifested by recurrent episodes of scaly, red, itchy skin patches that occur within apparently normal skin.

Objectives: This study was performed to detect the expression of serum and tissue (lesion and non-lesion) LncRNA MALAT-1 and MiRNA-9 that might be used as biomarkers for psoriasis.

Methods: Blood samples were obtained from 60 psoriasis patients and 40 controls, as well as 4 mm punch biopsy from lesional and non lesional skin of psoriatic patient and normal skin of healthy controls. Expression of LncRNA MALAT-1 and miRNNA-9 in serum and tissues was detected by real time qRT-PCR.

Results: a statistically significant increase in the expression of MALAT-1 in lesional and non-lesional skin and serum of psoriatic patients in comparison to controls were detected. Moreover, there was statistically significant increase in serum MiRNA-9 in patients in comparison to controls, while its tissue level was significantly lower in patients.

Conclusion: This study highlights the dysregulation of LncRNA MALAT-1 and miRNA-9 in psoriasis. Elevated expression of MALAT-1 in lesional skin of psoriatic patients compared to non-lesional skin may possibly contribute to the development of psoriatic plaques.

Keywords: MALAT-1; MiRNA-9; PCR; Psoriasis.