Fibronectin 1: A Potential Biomarker for Ovarian Cancer

Dis Markers. 2021 May 22:2021:5561651. doi: 10.1155/2021/5561651. eCollection 2021.

Abstract

Methods: OVCAR3 and A2780 are the two common cell lines that are used for ovarian cancer studies. The different invasion and migration abilities were observed by scratch tests and transwell experiments in our preliminary study. Gene chip was used to screen the expression gene in these two different cell lines, and then, the differentially expressed genes (at least 2-fold difference, P value < 0.05) were analyzed using KEGG.

Result: Fibronectin 1 (FN1) was found to be the most strongly correlated with the invasion and migration abilities of the OVCAR3 cells. Real-time PCR and FN1 knockout cell line was conducted and confirmed this finding. Based on the Oncomine database analysis, comparing with normal people, ovarian cancer patients exhibited high levels of FN1 expression. Additionally, higher FN1 expression was found in patients with higher FIGO stages of cancer.

Conclusion: FN1 could be a new biomarker for ovarian cancer detection and progress indicator.

MeSH terms

  • Adenocarcinoma / diagnosis*
  • Adenocarcinoma / genetics
  • Adenocarcinoma / metabolism
  • Adenocarcinoma / pathology
  • Biomarkers, Tumor / genetics
  • Biomarkers, Tumor / metabolism*
  • Blotting, Western
  • Cell Line, Tumor
  • Cell Movement
  • Databases, Factual
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Fibronectins / genetics
  • Fibronectins / metabolism*
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Neoplasm Invasiveness
  • Neoplasm Staging
  • Oligonucleotide Array Sequence Analysis
  • Ovarian Neoplasms / diagnosis*
  • Ovarian Neoplasms / genetics
  • Ovarian Neoplasms / metabolism
  • Ovarian Neoplasms / pathology
  • Real-Time Polymerase Chain Reaction

Substances

  • Biomarkers, Tumor
  • FN1 protein, human
  • Fibronectins