Rapid detection of chlorpyrifos residue in rice using surface-enhanced Raman scattering coupled with chemometric algorithm

Abstract

Due to the continuous development and progress of society and more and more attention to the quality and safety of food, rapid testing of pesticides in food is of great significance. In this paper, surface-enhanced Raman spectroscopy (SERS) and chemometric algorithms were employed collectively to quantify chlorpyrifos (CP) residues in rice samples. The SERS spectra from different concentrations (0.01-1000 μg/mL) of CP were collected using AgNPs-deposited-ZnO nanoflower (NFs)-like nanoparticles (Ag@ZnO NFs) SERS sensor. Four quantitative chemometric models for CP were comparatively studied, and the competitive adaptive reweighted sampling-partial least squares model achieved the best prediction and practical applicability for predicting CP levels with a limit of detection of 0.01 µg/mL. The results of the student's t-test showed no significant difference between this method and high-performance liquid chromatography (HPLC), and good relative standard deviation (RSD) indicated that this method could be used for the detection of CP in rice.

Keywords: Chemometric algorithms; Chlorpyrifos; Rice; Surface-enhanced Raman spectroscopy.