Four novel HLA class II alleles identified using next-generation sequencing

Alison Gareau; Ahmad Abu-Khader; Guang Yang; Luz Stamm; Noureddine Berka

doi:10.1111/tan.14338

Four novel HLA class II alleles identified using next-generation sequencing

HLA. 2021 Sep;98(3):233-234. doi: 10.1111/tan.14338. Epub 2021 Jun 15.

Authors

Alison Gareau^{1

2}, Ahmad Abu-Khader^{1

2}, Guang Yang¹, Luz Stamm¹, Noureddine Berka^{1

2}

Affiliations

¹ Histocompatibility and Immunogenetics Laboratory, Alberta Precision Laboratories, Calgary, Alberta, Canada.
² Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada.

PMID: 34089244
DOI: 10.1111/tan.14338

Abstract

We describe here four novel HLA class II alleles, DPA1*01:33, DPB1*1082:01, DPB1*1144:01, and DQA1*01:45, identified in our laboratory following the implementation of next generation sequencing (NGS) for routine high resolution HLA typing of donors and recipients for solid organ transplant and hematopoetic stem cell transplant. The NGS technology has improved our ability to match at all clinically HLA loci and to facilitate the interpretation of allele specific antibody and eplet matching.

Keywords: CLASS II; NGS; novel alleles.

MeSH terms

Alleles
HLA Antigens
High-Throughput Nucleotide Sequencing*
Histocompatibility Testing
Organ Transplantation*

Substances

HLA Antigens