Hydrogen sulfide (H2S) underpins the beneficial silicon effects against the copper oxide nanoparticles (CuO NPs) phytotoxicity in Oryza sativa seedlings

Abstract

Nanoparticle-pollution has associated severe negative effects on crop productivity. Hence, methods are needed to alleviate nano-toxicity in crop plants. The present study aims to evaluate if the exogenous hydrogen sulfide (H₂S) application in combination with silicon (Si) could palliate the harmful effects of copper oxide nanoparticles (CuO NPs). Fifteen day-old rice (Oryza sativa L.) seedlings were used as a model plant. The results indicate that simultaneous exogenous addition of 10 μM Si and 100 μM NaHS (as an H₂S donor) provided tolerance and enhanced defence mechanism of the rice seedlings against 100 μM CuO NPs. Thus, it was observed in terms of their growth, photosynthetic pigments, antioxidant enzyme activities, the content of non-enzymatic components, chlorophyll fluorescence and up-regulation of antioxidant genes. Si and NaHS stimulated gene expression of silicon (Lsi1 and Lsi2) and auxin (PIN5 and PIN10) transporters. Taken together, data indicate that H₂S underpins the beneficial Si effects in rice seedlings against the oxidative stress triggers by CuO NPs, and stimulation of enzymatic components of the ascorbate-glutathione cycle being the main factor for the beneficial effects triggered by the couple of Si and H₂S. Therefore, it could be concluded that the simultaneous application of Si and H₂S promote the resilience of the rice seedlings against the oxidative stress induced by CuO NPs.

Keywords: (Sodium hydrogen sulphide (NaHS); Antioxidant enzymes; Copper oxide nanoparticles (CuO NPs); Hydrogen sulphide (H(2)S); Reactive oxygen species (ROS); Rice seedlings; Silicon; Toxicity.