The thermal regime modifies the response of aquatic keystone species Daphnia to microplastics: Evidence from population fitness, accumulation, histopathological analysis and candidate gene expression

Abstract

The water bodies are greatly influenced by chemical contamination and global increasing temperature. As an emerging pollutant, microplastics are widely distributed in the freshwater environment, raising concerns regarding their potential toxicity to organisms. Especially for zooplankton filter feeders, many of microplastics are in similar size as their food. Individually, both microplastics and temperature have profound effects on zooplankton populations and their function in ecosystems. However, the strength and direction of their interactive effects are still not clear. Here, we performed a comprehensive biotoxicity assessment providing empirical evidence that the temperature played a key role in shaping the sensitivity of the zooplankter, Daphnia magna, against microplastic toxicity. We found that exposure to microplastics generally caused negative effects on Daphnia individual fitness, such as increased lethality, declined fecundity and reduced population growth rate. This microplastic toxicity was more prominent at 30 °C than at 20 °C, and was rather minor at 15 °C. Moreover, the warming accelerated the ingestion of microplastics, and triggered abnormal ultrastructure of intestinal epithelial cells. In addition, the expression profiling of candidate genes revealed oxidative damage, fecundity impairment and energy retardation by microplastics were amplified with increasing temperature, which may contribute to the enhancement of microplastic toxicity under warming. Given that high temperature fluctuations are becoming more common and difficult to predict, the interactive effects of microplastics and climate warming on Daphnia population dynamics and biomass production may become increasingly aggravated in nature. Collectively, extrapolation for environmental risk assessment studies conducted under different temperature contexts may broaden our knowledge microplastic toxicity on aquatic organism fitness.

Keywords: Climate warming; Molecular toxicology; Plastic pollution; Population fitness; Zooplankton.